Short Description
Quantifies salicylate concentration using a colorimetric (610 nm) method.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Salicylate Assay Kit provides a rapid, reliable solution for quantifying salicylate levels in biological fluids and consumer products. This high-throughput compatible system utilizes a simple "add-mix-read" procedure, measuring stable color complexes at 560 nm. It is essential for pre-clinical toxicity screening and monitoring aspirin metabolism in pharmaceutical research.
Features
Rapid Throughput & Precision: Utilizes a streamlined "mix-incubate-read" protocol, providing high-sensitivity quantification within 20 minutes for large-scale sample sets.
Minimal Sample Interference: Engineered with specific enzyme catalysts to ensure high selectivity, effectively eliminating cross-reactivity from common endogenous metabolic compounds.
Extended Linear Range: Offers a broad detection dynamic, ensuring accurate measurement from low-dose pharmacological studies to high-range toxicological evaluations.
Versatile Platform Compatibility: Formatted for standard 96-well plates, facilitating easy integration into automated liquid handling systems and various spectrophotometric readers.
Process Relevance
Monitoring salicylate levels to ensure therapeutic safety, assess metabolic clearance, and optimize drug formulation stability.
Application Stage
Toxicological screening, pharmacokinetic profiling, and quality control during pharmaceutical manufacturing.
Applications
Quantifying salicylate in serum, plasma, and urine; supporting monitoring of aspirin metabolism, drug-drug interaction studies, and acute poisoning diagnostics in research and preclinical settings.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Direct quantification of salicylate (ortho-hydroxybenzoic acid) concentrations in biological fluid samples.
Detection Method
Absorbance at 560 nm
Sample Type
Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc.
Research Areas
Pharmacokinetics (ADME), preclinical toxicology, cardiovascular research, inflammatory response, drug safety assessment, etc.
Datasheet