OXPHOSight™ Salicylate Assay Kit

CAT#: ITS-1125-WB2
Product Type: Assay Kit
Species: All Species
Target: Direct quantification of salicylate (ortho-hydroxybenzoic acid) concentrations in biological fluid samples.
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies salicylate concentration using a colorimetric (610 nm) method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Salicylate Assay Kit provides a rapid, reliable solution for quantifying salicylate levels in biological fluids and consumer products. This high-throughput compatible system utilizes a simple "add-mix-read" procedure, measuring stable color complexes at 560 nm. It is essential for pre-clinical toxicity screening and monitoring aspirin metabolism in pharmaceutical research.
Features
Rapid Throughput & Precision: Utilizes a streamlined "mix-incubate-read" protocol, providing high-sensitivity quantification within 20 minutes for large-scale sample sets.
Minimal Sample Interference: Engineered with specific enzyme catalysts to ensure high selectivity, effectively eliminating cross-reactivity from common endogenous metabolic compounds.
Extended Linear Range: Offers a broad detection dynamic, ensuring accurate measurement from low-dose pharmacological studies to high-range toxicological evaluations.
Versatile Platform Compatibility: Formatted for standard 96-well plates, facilitating easy integration into automated liquid handling systems and various spectrophotometric readers.
Process Relevance
Monitoring salicylate levels to ensure therapeutic safety, assess metabolic clearance, and optimize drug formulation stability.
Application Stage
Toxicological screening, pharmacokinetic profiling, and quality control during pharmaceutical manufacturing.
Applications
Quantifying salicylate in serum, plasma, and urine; supporting monitoring of aspirin metabolism, drug-drug interaction studies, and acute poisoning diagnostics in research and preclinical settings.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Direct quantification of salicylate (ortho-hydroxybenzoic acid) concentrations in biological fluid samples.
Detection Method
Absorbance at 560 nm
Sample Type
Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc.
Research Areas
Pharmacokinetics (ADME), preclinical toxicology, cardiovascular research, inflammatory response, drug safety assessment, etc.
Size
100 Tests
Data Output Type
Quantitative salicylate concentration measurement (colorimetric endpoint at 560 nm-585 nm).
Assay Time
30 min
Species
All Species
Storage
Store at 4 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Do not freeze. Keep components away from direct sunlight and avoid prolonged exposure to room temperature during use. Ensure containers are tightly sealed after each use to prevent evaporation or contamination. Return all components promptly to 4 ℃ storage after handling.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Salicylate, the primary metabolite of aspirin, modulates cyclooxygenase activity to manage pain and inflammation. Precise monitoring is critical due to its narrow therapeutic index and potential for dose-dependent toxicity.
Pathways
Salicylate inhibits NF-κB and COX pathways to attenuate pro-inflammatory cytokine production. It is metabolized primarily in the liver through conjugation with glycine or glucuronic acid for renal excretion.
For Research Use Only | Not For Clinical Use
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