OXPHOSight™ Sorbitol Assay Kit

CAT#: ITS-1125-WB39
Product Type: Assay Kit
Species: All Species
Target: Specifically detects D-Sorbitol concentration via enzymatic oxidation coupled with colorimetric or fluorometric signal output.
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies sorbitol concentration using a colorimetric (565 nm) or fluorimetric (530/585 nm) method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Sorbitol Assay Kit provides a sensitive and reliable platform for quantifying sorbitol concentrations in biological, food, and beverage samples. This high-throughput compatible system utilizes an enzyme-coupled MTT/NAD reaction, making it essential for monitoring metabolic pathways and diabetes progression in pre-clinical research. By offering a simple, non-radioactive colorimetric method, it supports efficient drug screening and metabolic profiling to optimize therapeutic development.
Features
Rapid High-Throughput Format: Employs a simplified "mix-incubate-measure" protocol compatible with 96-well plates, ensuring high efficiency for large-scale screenings.
Superior Detection Sensitivity: Utilizes advanced enzymatic oxidation to achieve low micromolar detection limits across diverse biological and food matrices.
Exceptional Substrate Specificity: Minimizes interference from other sugars through optimized enzyme-coupled reactions, providing highly accurate and reproducible sorbitol measurements.
Process Relevance
Ensuring precise metabolic flux monitoring to optimize fermentation yields and maintain consistent quality in biopharmaceutical manufacturing processes.
Application Stage
Ideal for drug candidate screening, metabolic pathway development, and rigorous in-process industrial quality control validation.
Applications
Quantifying sorbitol in serum, plasma, urine, and diverse food samples; supports diabetes research, polyol pathway analysis, and industrial sugar-free product verification.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Specifically detects D-Sorbitol concentration via enzymatic oxidation coupled with colorimetric or fluorometric signal output.
Detection Method
Absorbance at 565 nm
Sample Type
Biological (e.g. blood), food, beverage, and agriculture samples
Research Areas
Metabolism & endocrinology, diabetes research, food & beverage science, clinical diagnostics, polyol pathway regulation, etc.
Size
100 tests
Data Output Type
Quantitative sorbitol concentration measurement via colorimetric (565 nm) or fluorometric (Ex/Em = 530/585 nm) endpoint analysis.
Assay Time
30 min
LOD
5 µM
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Sorbitol, a sugar alcohol produced via glucose reduction, is a critical biomarker for osmotic stress and diabetic complications. Its accurate quantification is essential for understanding metabolic shifts and cellular pathology.
Pathways
Involved primarily in the polyol pathway, where glucose is reduced to sorbitol by aldose reductase. Excess sorbitol accumulation triggers oxidative stress and downstream complications in hyperglycemic conditions.
For Research Use Only | Not For Clinical Use
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