Short Description
Bradford-based quantification of total protein.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Total Protein Assay Kit utilizes the Coomassie Blue (Bradford) method, essential for normalizing enzyme activity data in lysate samples. This assay provides a rapid and robust measure of protein concentration, supporting accurate specific activity calculations in drug discovery. It is a fundamental tool for standardization in biochemical assays and proteomic workflows.
Features
Coomassie Blue (Bradford) method; Essential for normalizing enzyme activity data in lysate samples.
Process Relevance
Sample Normalization
Application Stage
Research / All Stages
Applications
Normalization of enzymatic data and purity checking.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 600 nm (Pyrogallol Red)
Sample Type
Urine, cerebrospinal fluid, samples
Research Areas
General Laboratory; Clinical Chemistry
Datasheet