The OXPHOSight™ Total Protein Assay Kit utilizes the Coomassie Blue (Bradford) method, essential for normalizing enzyme activity data in lysate samples. This assay provides a rapid and robust measure of protein concentration, supporting accurate specific activity calculations in drug discovery. It is a fundamental tool for standardization in biochemical assays and proteomic workflows.
Features
Coomassie Blue (Bradford) method; Essential for normalizing enzyme activity data in lysate samples.
Process Relevance
Sample Normalization
Application Stage
Research / All Stages
Applications
Normalization of enzymatic data and purity checking.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 600 nm (Pyrogallol Red)
Sample Type
Urine, cerebrospinal fluid, samples
Research Areas
General Laboratory; Clinical Chemistry
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 10 min
LOD
5 mg/dL
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid freezing unless otherwise specified. During use, minimize prolonged exposure to room temperature and return components promptly to 2-8 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Total protein concentration in serum reflects nutritional status and liver synthetic capacity. Deviations from normal ranges can indicate dehydration liver disease or kidney dysfunction.