Short Description
Quantifies ADP using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Two-Step ADP Assay Kit provides a rapid colorimetric method for quantifying ADP levels in biological samples through a coupled enzymatic reaction. The system measures the decrease in NADH, which correlates directly with the ADP concentration, offering a robust approach for metabolic monitoring. This reliable tool facilitates research into energy transduction and enzyme kinetics, ensuring consistent performance for fundamental studies in biochemistry and cellular metabolism.
Features
Features an ultra-sensitive fluorimetric detection system for ADP quantification, providing enhanced resolution for mitochondrial metabolism studies and high-throughput enzyme inhibitor screening in research.
Process Relevance
Quantifying ADP to characterize enzymatic reaction rates and evaluate energy utilization in biological models.
Application Stage
Metabolic flux analysis and cellular signaling research.
Applications
Measure ADP production in enzymatic assays and cell lysates to evaluate metabolic activity and nucleotide turnover in various models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Luminescence
Sample Type
Cell lysates, tissue homogenates, biological fluids.
Research Areas
Bioenergetics; Cell Metabolism; Enzyme Kinetics
Datasheet