OXPHOSight™ Two-Step ADP Assay Kit

CAT#: ITS-1125-YL23
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies ADP using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Two-Step ADP Assay Kit provides a rapid colorimetric method for quantifying ADP levels in biological samples through a coupled enzymatic reaction. The system measures the decrease in NADH, which correlates directly with the ADP concentration, offering a robust approach for metabolic monitoring. This reliable tool facilitates research into energy transduction and enzyme kinetics, ensuring consistent performance for fundamental studies in biochemistry and cellular metabolism.
Features
Features an ultra-sensitive fluorimetric detection system for ADP quantification, providing enhanced resolution for mitochondrial metabolism studies and high-throughput enzyme inhibitor screening in research.
Process Relevance
Quantifying ADP to characterize enzymatic reaction rates and evaluate energy utilization in biological models.
Application Stage
Metabolic flux analysis and cellular signaling research.
Applications
Measure ADP production in enzymatic assays and cell lysates to evaluate metabolic activity and nucleotide turnover in various models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Luminescence
Sample Type
Cell lysates, tissue homogenates, biological fluids.
Research Areas
Bioenergetics; Cell Metabolism; Enzyme Kinetics
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
20 min
LOD
LOD: 0.02 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Reliable measurement of ADP levels is necessary for characterizing the activity of ATP-consuming enzymes. Maintaining stability and minimizing the spontaneous hydrolysis of ATP are key challenges in these types of assays.
Pathways
Hydroxyproline is produced by the post-translational hydroxylation of proline within the collagen triple helix. It is a critical downstream marker of collagen synthesis and is essential for the thermal stability of the extracellular matrix.
For Research Use Only | Not For Clinical Use
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