Pig LGALS9 ELISA Kit, Lot 21AO-218 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Pig LGALS9 in Cell Culture Supernatant, Plasma, Serum.

Porcine Immunoquantitative (PCR-Based) Galectin-9 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELISA

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

The Immuno-Quantitative ELISA kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, quantitative ELISA enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

LGALS9

Pig

qPCR

Sandwich ELISA

Semi-Quantitative

Cell Culture Supernatant, Plasma, Serum

Galectin-9 Microplate (Item A): 96 well PCR plate coated with anti-Porcine Galectin-9.
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
Standards (Item C): 2 vials of recombinant Porcine Galectin-9.
Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative.
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer.
Detection Affinity Reagent for Galectin-9 (Item F): 2 vials of a 4x concentrated solution of anti-Porcine Galectin-9 affinity reagent.
Quantitative ELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock.
Primer Solution (Item I): 1.7 ml vial.
PCR Master Mix (Item J): 1.2 ml vial.
PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer.
Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer.

Real-time PCR instrument, Bio-Rad recommended.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
100 mL and 1 liter graduated cylinders.
Absorbent paper.
Distilled or deionized water.
Log-log graph paper or computer and software for data analysis.
Tubes to prepare standard or sample dilutions.
Heating block or water bath capable of 80°C.

25 μL

Pre-coated

4 °C,-20 °C,-80 °C

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

6 months

May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

For Research Use only

Galectin 9 (GAL9)

huat; lgals9a; ecalectin; galectin-9; Lgals9; wu:fd20c09; zgc:111833; LGALS9; AA407335; AI194909; AI265545; LGALS35; Lgals5; gal-9; HUAT; LGALS9A; UAT; UATP.I; galectin 9 L homeolog; galectin 9; lectin; galactoside-binding; soluble; 9 (galectin 9)-like 3; lectin; galactose binding; soluble 9; lgals9.L; lgals9; lgals9l3; LGALS9; Lgals9

1.Prepare all reagents, samples and standards as instructed.
2.Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C.
3.Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature.
4.Add 25μL of quantitative ELISA Detection Reagent to each well. Incubate 1 hour.
5.Add 15μL Primer solution 10μL of PCR master mix to each well.
6.Run real-time PCR.