Rabbit HMGB1 ELISA Kit, Lot 21AO-431 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P342
Product Type: ELISA Kit
Target: HMGB1
Short Description
The kit is designed for in vitro quantitative measurement of Rabbit HMGB1 in Plasma, Serum, Tissue Homogenate.
Description
For quantitative detection of HMGB-1 in serum, plasma, tissue homogenates.
Applications
ELISA
Target
HMGB1
Reactivity
Rabbit
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of HMGB-1. No significant cross- reactivity or interference between HMGB-1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between HMGB-1 and all the analogues, therefore, cross reaction may still exist.
Components
Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Sample/Standard Dilution Buffer
Biotin-labeled Antibody (Concentrated)
Antibody Dilution Buffer
HRP-Streptavidin Conjugate (SABC)
SABC Dilution Buffer
TMB Substrate
Stop Solution
Wash Buffer (25 x concentrate)
Instruction manual
Material not included
1.Microplate reader (wavelength:450nm)
2.37 °C incubator
3.Automated plate washer
4.Precision single and multi-channel pipette and disposable tips
5.Clean tubes
6.Deionized or distilled water
Sensitivity
0.375 ng/mL
Sample Volume
100 μL
Plate
Pre-coated
Reagent Preparation
Bring all reagents and samples to room temperature for 20 minutes before use.
Wash Buffer
Standards
Preparation of Biotin-labeled Antibody Working Solution
Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution
Assay Procedure
Set standard, test samples (diluted at least 1/2 with Sample Dilution Buffer), control (blank) wells on the pre-coated plate respectively, and then, records their positions. Wash plate 2 times before adding standard, sample and control (blank) wells.
Prepare Standards
Add Samples
Incubate
Wash
Biotin-labeled Antibody
Wash
HRP-Streptavidin Conjugate (SABC)
Wash
TMB Substrate
Stop
OD Measurement
Assay Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Storage
4 °C,-20 °C
Storage Comment
For unused kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon receipt while the others should be at 4 °C.
For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
Expiry Date
6 months
Note
For unused kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon receipt while the others should be at 4 °C.
For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
Restrictions
For Research Use only
Alternative Name
High Mobility Group Protein 1 (HMGB1)
Synonyms
HMG1; HMG3; SBP-1; DEF; HMG-1; Hmg1; amphoterin; p30; hmgb1; ik:tdsubc_1a5; wu:fb23c02; xx:tdsubc_1a5; zgc:56110; zgc:77104; hmg-1; hmg3; sbp-1; hmg1; HMGB1; Ac2-008; high mobility group box 1; high-mobility group box 1; high mobility group box 1a; high mobility group box 1 L homeolog; high mobility group protein B1; HMGB1; Hmgb1; hmgb1; hmgb1a; hmgb1.L; LOC100359149
Background
HMGB1, High mobility group protein B1, HMG-1, HMG1, HMG3, SBP-1, Amphoterin, high mobility group box 1, High mobility group protein 1, high-mobility group, nonhistone chromosomal protein 1, high-mobility group box 1, Sulfoglucuronyl carbohydRate binding protein
UniProt
B7NZ89
Pathways
p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Protocol
1.Wash plate 2 times before adding Standard, Sample and Control wells!
2.Add 100 µL standard or sample to each well and incubate for 90 minutes at 37 °C.
3.Aspirate and wash plates 2 times.
4.Add 100 µL Biotin-labeled antibody working solution to each well and incubate for 60 minutes at 37 °C.
5.Aspirate and wash plates 3 times.
6.Add 100 µL SABC Working Solution into each well and incubate for 30 minutes at 37 °C.
7.Aspirate and wash plates 5 times.
8.Add 90 µL TMB Substrate Solution. Incubate 10-20 minutes at 37 °C.
9.Add 50 µL Stop Solution. Read at 450nm immediately and calculation.
For Research Use Only | Not For Clinical Use
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