Rat CD276 ELISA Kit, Lot 21AO-1259 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Rat CD276 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

Rat B7-H3/CD276 ELISA Kit.

ELISA

Optimal working dilution should be determined by the investigator.

CD276

Rat

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Pre-coated

6 months

For Research Use only

Cluster Of Differentiation 276 (CD276)

sc:d0645; zgc:158279; 6030411F23Rik; AU016588; B7RP-2; B7h3; B7H3; 4Ig-B7-H3; B7-H3; CD276 molecule; CD276 molecule S homeolog; CD276 antigen; Cd276 molecule; CD276; cd276.S; cd276; Cd276

315716

Q7TPB4

Cancer Immune Checkpoints

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.