Rat CD80 ELISA Kit, Lot 21AO-186 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P097
Product Type: ELISA Kit
Target: CD80
Short Description
The kit is designed for in vitro quantitative measurement of Rat CD80 in Cell Culture Supernatant, Serum.
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD80/B7-1.
Applications
ELISA
Application Notes
Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Target
CD80
Reactivity
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum
Specificity
Expression system for standard: NSO; Immunogen sequence: I39-Q248
Cross-Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Binding Specificity
AA 39-248
Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Sensitivity
<2pg/mL
Plate
Pre-coated
Assay Procedure
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well.
Assay Precision
Sample 1: n=16, Mean(ng/ml): 0.28, Standard deviation: 0.015, CV(%): 5.2
Sample 2: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.111, CV(%): 7.1
Sample 3: n=16, Mean(ng/ml): 2.31, Standard deviation: 0.113, CV(%): 4.9
Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.016, CV(%): 6.2
Sample 2: n=24, Mean(ng/ml): 1.72, Standard deviation: 0.138, CV(%): 8.0
Sample 3: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.134, CV(%): 5.4
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage
-20 °C,4 °C
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date
12 months
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only
Alternative Name
B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Synonyms
B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80 molecule; Cd80 molecule; CD80 antigen; CD80; Cd80
Gene ID
25411
UniProt
G3V671
Pathways
TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Protocol
A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, I39-Q248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
For Research Use Only | Not For Clinical Use
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