Rat Endotoxin ELISA Kit (Sandwich)-96 tests (CAT#: IO-HMM73)

96 tests

Pre-coated 96-well microplate; Standard; Standard diluent buffer; Wash buffer; Detection reagent A; Detection reagent B; Diluent A; Diluent B; TMB substrate; Stop solution; Plate sealer

< 0.17 ng/mL

1) Set standard, test samples and control wells.
2) Aliquot 50 µl of diluted standard into the standard wells.
3) Aliquot 50 µl of Standard Diluent buffer into the control (zero) well.
4) Aliquot 50 µl of diluted samples into the sample wells.
5) Immediately aliquot 50 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
6) Wash 3 times.
7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
8) Wash 5 times.
9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
10) Aliquot 50 µl of Stop Solution.
11) Measure the OD at 450 nm.

4°C