Rat ICAM1 ELISA Kit, Lot 21AO-873 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P784
Product Type: ELISA Kit
Target: ICAM-1
Short Description
The kit is designed for in vitro quantitative measurement of Rat ICAM1 in Cell Culture Supernatant, Plasma (EDTA - heparin), Serum.
Description
Sandwich ELISA for Quantitative Detection of Antigen
Applications
ELISA
Application Notes
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat ICAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.
Blood Product Anticoagulant: Heparin Sodium.
ELISA Dilution: 31.2pg/mL-2000pg/mL.
Target
ICAM-1
Reactivity
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Sample Volume
100 μL
Plate
Pre-coated
Storage
RT,4 °C,-20 °C
Storage Comment
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Note
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
Restrictions
For Research Use only
Alternative Name
ICAM-1/CD54
Synonyms
BB2; CD54; P3.58; Icam-1; Ly-47; MALA-2; ICAM; ICAM-1; ICAM1; intercellular adhesion molecule 1; intercellular adhesion molecule-1; ICAM1; Icam1; ICAM-1
Gene ID
25464
UniProt
Q00238
Pathways
Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
For Research Use Only | Not For Clinical Use
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