Rat IDO ELISA Kit, Lot 21AO-1073 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P984
Product Type: ELISA Kit
Target: IDO
Short Description
The kit is designed for in vitro quantitative measurement of Rat IDO in Cell Lysate, Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of rat indoleamine 2, 3-dioxygenase (IDO) concentrations in serum, plasma, tissue homogenates, cell lysates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
IDO
Reactivity
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Lysate, Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of rat IDO. No significant cross-reactivity or interference between rat IDO and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat IDO and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
7.81 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C,-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C.
Restrictions
For Research Use only
Alternative Name
Indoleamine 2,3-Dioxygenase
Synonyms
IDO; IDO-1; INDO; Ido; Indo; indoleamine 2;3-dioxygenase 1; IDO1; Ido1
Background
Abbreviation: IDO1
Alias: CD107B, IDO, INDO, indole 2,3-dioxygenase|indoleamine-pyrrole 2,3 dioxygenase
Gene ID
66029
UniProt
Q9ERD9
Pathways
Activated T Cell Proliferation
For Research Use Only | Not For Clinical Use
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