Rat Prostaglandin E2 (PGE2) ELISA Kit, Lot 21AO-657 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Rat Prostaglandin E2 (PGE2) in Serum, Plasma, Tissue Homogenate, Cell Lysate.

For the quantitative determination of endogenic rat prostaglandin E2(PG-E2) concentrations in serum, plasma, tissue homogenates, cell lysates.

ELISA

Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
Grossly hemolyzed samples are not suitable for use in this assay.
If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
Please predict the concentration before assaying.
Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
Fresh samples without long time storage are recommended for the test.

Detection wavelength: 450 nm
Information on standard material: Depending on the antigen to be detected, standards can be either native or recombinant protein.
Information on reagents: In most cases the stop solution provided is 1 N H2SO4.
Information on antibodies: The antibodies provided in different kits vary in regards to clonality and host.

Prostaglandin E2 (PGE2)

Rat

Colorimetric

Competition ELISA

Quantitative

Serum, Plasma, Tissue Homogenate, Cell Lysate

This assay has high sensitivity and excellent specificity for detection of rat PGE2.

Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Assay plate (12 × 8 coated Microwells)
Standard (freeze dried)
Biotin-antibody (100 × concentrate)
HRP-avidin (100 × concentrate)
Biotin-antibody Diluent
HRP-avidin Diluent
Sample Diluent
Wash Buffer (25 × concentrate)
TMB Substrate
Stop Solution
Adhesive Strip (for 96 wells)
Instruction manual

Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540nm or 570nm.
An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.
Squirt bottle, manifold dispenser or automated microplate washer.
Absorbent paper for blotting the microtiter plate.
100mL and 500mL graduated cylinders.
Deionized or distilled water.
Pipettes and pipette tips.
Test tubes for dilution.

0.25 pg/mL

50 μL

1 - 4.5 h

Pre-coated

Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit.

Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
Intra-assay: CV% less than 8%
Inter-assay: CV% less than 10%

The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

4 °C/-20 °C

May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.

6 months

May be stored at 2-8°C for up to 1 month. For long term storage, please store at -20°C. Try to keep assay plate in a sealed aluminium foil bag and avoid dampness.

For Research Use only

Prostaglandin E2 (PG-E2)

The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PGE2 and Horseradish Peroxidase (HRP) conjugated PGE2. The competitive inhibition reaction is launched between with HRP labeled PGE2 and unlabeled PGE2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PGE2 in the sample.