Rat Tumor Necrosis Factor (TNF) ELISA Kit, Lot 21AO-135 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P046
Product Type: ELISA Kit
Target: Tumor Necrosis Factor (TNF)
Short Description
The kit is designed for in vitro quantitative measurement of Rat Tumor Necrosis Factor (TNF) in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA).
Applications
ELISA
Application Notes
Detection Wavelength: 450 nm
Target
Tumor Necrosis Factor (TNF)
Reactivity
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
Specificity
Natural and recombinant Rat TNF-α Ligand
Material not included
Microplate reader.
Pipettes and pipette tips.
EP tube Deionized or distilled water.
Sensitivity
15 pg/mL
Sample Volume
20 μL
Assay Time
3 h
Plate
Pre-coated
Storage
4 °C
Restrictions
For Research Use only
Alternative Name
Tumor Necrosis Factor Alpha (TNFa)
Synonyms
DIF; TNF-alpha; TNFA; TNFSF2; RATTNF; Tnfa; tnf; TNF-a; TNFalpha; Tnfsf1a; TNFa; cTNF; Tnf-alpha; tnfa-like; TNF-ALPHA; dif; tnfa; xtnf; tnfsf2; tnf-alpha; Cachectin; tumor necrosis factor; tumor necrosis factor b (TNF superfamily; member 2); tumor necrosis factor alpha; tumor necrosis factor a (TNF superfamily; member 2); TNF; Tnf; tnf; tnfb; tnf-alpha; LOC103694380; tnfa
Background
Rat TNF-α cDNA encodes a 235 amino acid (aa) residue type II membrane protein. The 156 aa residue soluble TNF-αis released from the C-terminus of the membrane-anchored TNF-α by TNF-α-converting enzyme (TACE), a matrix metalloprotease.The membrane-anchored form of TNF-α has been shown to have lytic activity and may also have an important role in intercellular communication.
Gene ID
24835
UniProt
P16599
Pathways
NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
For Research Use Only | Not For Clinical Use
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