Rat VEGF ELISA Kit, Lot 21AO-950 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Rat VEGF in Plasma, Cell Culture Supernatant, Serum, Tissue Samples.

ELISA

VEGF

Rat

Colorimetric

Sandwich ELISA

Quantitative

Plasma, Cell Culture Supernatant, Serum, Tissue Samples

Aluminium pouches with a Microwell Plate: 8x12
Standard: 2xVial Biotinylated antibody: 2xVial
Streptavidin-HRP: 2xVial
Assay Buffer (25ml/Vial): 2xVial
Wash Buffer Concentrate 20x (30ml/Vial): 1xVial
TMB Substrate Solution: 1x12ml
Stop Solution (12ml/Vial): 1xVial
Plate Covers- Adhesive strips: 3xstrips
Instruction: 1x

4 pg/mL

Pre-coated

4 °C,-20 °C

4 °C,-20 °C

4 °C,-20 °C

For Research Use only

Vascular Endothelial Cell Growth Factor

MVCD1; VEGF; VPF; Vegf; Vegf120; Vegf164; Vegf188; Vpf; VEGF-A; VEGF164; eVEGF120; eVEGF164; vegf-a; vegf; vascular endothelial growth factor A; vascular endothelial growth factor A L homeolog; vascular endothelial growth factor; vascular endothelial growth factor precursor; VEGFA; Vegfa; vegfa.L; VEGF; vegf

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 42kD, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells.

83785

P16612

RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

This assay employs the Sandwich immunoassay technique. An anti r VEGF monoclonal coating antibody is adsorbed onto microwells. VEGF present in the sample or standard and Biotinylated anti VEGF antibody binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard and Biotinylated anti VEGF antibody are removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti VEGF antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of VEGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm.