SHP-77 In Vitro BrdU Assay (Cell Proliferation)

This assay is to provide SHP-77-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

SHP-77

Human

SHP-77 epithelial cells are derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung of a 54-year-old, White male in 1977. SHP-77 cells can serve as an in vitro target in 51Cr and 111 in release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients. The cells can be used to evaluate the immune status of patients with SCLC who are treated with radiation or chemotherapy.

Lung Cancer

Oncology

In Vitro BrdU Assay (Cell Proliferation)

5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent sig nal on DNA synthesis.

5-Bromo-2'-deoxyuridine (BrdU) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis