SHP-77 In Vitro Trypan Blue Exclusion Assay (Cell Viability)
CAT#: ITS-0123-YF2431
Target Cell Organism: Human
Target Cell Name: SHP-77
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide SHP-77-based In Vitro Trypan Blue Exclusion Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
SHP-77
Target Cell Organism
Human
Target Cell Background
SHP-77 epithelial cells are derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung of a 54-year-old, White male in 1977. SHP-77 cells can serve as an in vitro target in 51Cr and 111 in release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients. The cells can be used to evaluate the immune status of patients with SCLC who are treated with radiation or chemotherapy.
Related Diseases
Lung Cancer
Research Area
Oncology
Assay Name
In Vitro Trypan Blue Exclusion Assay (Cell Viability)
Assay Description
The intact cell membranes cannot recruit these dyes into cells. Trypan blue can enter into non-viable cells, while viable cells exclude trypan blue. The trypan blue exclusion assay is a simple and commonly used assay for cell viability determination.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.