Short Description
Measures gene expression using a bioluminescent method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
This SigPath™ Dual Luciferase Reporter Gene Assay Kit provides a highly sensitive bioluminescent system for monitoring gene expression in cell-based research. By sequentially measuring firefly and renilla luciferase activities, the system allows for internal normalization and precise quantification of transcriptional regulation. This robust tool is essential for researchers studying promoter activity, signaling pathways, and gene silencing efficiency, ensuring high accuracy and reproducibility in molecular biology.
Features
Enables sequential dual-bioluminescent measurement for accurate gene expression normalization, providing superior sensitivity and dynamic range for promoter activity and transcriptional research.
Process Relevance
Normalizing gene expression data to ensure accurate assessment of promoter activity and cellular response.
Application Stage
Gene expression analysis and promoter activity screening.
Applications
Monitor promoter activity and normalize transfection efficiency in cell-based assays using bioluminescent reporters for gene regulation studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Luminescence
Research Areas
Gene Regulation; Cell Signaling; Molecular Biology
Datasheet