Short Description
Quantifies ERK1/2 phosphorylation levels in whole cells using an ELISA-based colorimetric method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ ERK Phosphorylation Assay Kit offers a streamlined, cell-based ELISA for quantifying ERK1/2 phosphorylation without the need for cell lysate preparation. This high-throughput system normalizes phosphorylation levels to total cellular protein, ensuring exceptional accuracy for screening kinase inhibitors and studying MAPK signaling. By reducing assay time to just 6.5 hours, it provides a rapid and robust tool for drug discovery and signal transduction research in pre-clinical studies.
Features
Dual-Signal Precision: Measures phosphorylated ERK and total protein concurrently in the same well to ensure robust, normalized data accuracy.
Streamlined Workflow: Eliminates time-consuming cell lysis and protein transfer steps, reducing total assay time to approximately 6.5 hours.
High-Throughput Compatibility: Optimized for 96-well formats, facilitating rapid screening of thousands of compounds with automated liquid handling systems.
Sensitive Fluorescence Detection: Utilizes high-performance immunoenzymatic labeling to detect subtle shifts in phosphorylation status across human, mouse, and rat species.
Process Relevance
Monitoring ERK activation levels to support high-throughput inhibitor screening and precise signal transduction pathway optimization during lead validation.
Application Stage
Target validation, secondary lead optimization, and high-throughput drug screening within the pharmaceutical research and development pipeline.
Applications
Designed for quantitative fluorescence immunoenzymatic determination of ERK1/2 phosphorylation in cultured cells to evaluate ligand efficacy, kinase inhibitor potency, and growth factor-induced signaling.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Phosphorylated ERK1 (T202/Y204) and ERK2 (T185/Y187) within intact, fixed cellular environments.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 451 nm)
Research Areas
Oncology therapeutics, cellular differentiation, drug discovery, immunology, signal transduction, etc.
Datasheet