SigPath™ Glutamine Assay Kit

CAT#: ITS-1125-HM26
Product Type: Assay Kit
Species: All Species
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies glutamine, the most abundant free amino acid.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Glutamine Assay Kit monitors nutrient utilization in bioreactors to optimize cell growth, critical for maximizing yield in monoclonal antibody production. It also supports the development of glutaminase inhibitors in cancer therapy by analyzing tumor metabolism. This cell-culture compatible format allows for the precise tracking of glutamine consumption, a primary fuel source for rapidly dividing cells.
Features
Cell-culture compatible format for monitoring nutrient consumption; Critical for optimizing yield in monoclonal antibody production and analyzing tumor metabolism.
Process Relevance
Bioprocess Nutrient Monitoring
Application Stage
Manufacturing (Upstream)
Applications
Monitors nitrogen transport and cellular growth in cell culture.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, plasma, urine, cell, tissue, samples
Research Areas
Oncology; Bioprocessing
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 40 min
LOD
23 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutamine is the most abundant free amino acid in the blood serving as a primary nitrogen donor and carbon source for rapidly dividing cells. It is a critical nutrient for immune cells and cancer cells which often exhibit glutamine addiction.
For Research Use Only | Not For Clinical Use
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