SigPath™ Human/Mouse GSK3Α(S21) Phosphorylation ELISA Kit (CAT#: ITS-1125-WB8)

Quantifies GSK3α (Ser21) phosphorylation levels in cell lysates using a colorimetric ELISA method.

SigPath

Research-Grade Signal Pathway Assay Development

The SigPath™ Human/Mouse GSK3Α(S21) Phosphorylation ELISA Kit provides a rapid, cell-based solution for quantifying GSK3Α phosphorylation at Serine-21 without the need for cell lysis. This high-throughput compatible system enables precise monitoring of signal transduction pathways and drug-induced effects by normalizing phosphorylated protein levels to total cellular protein. By simplifying workflows and reducing assay time, it facilitates efficient screening of kinase inhibitors and activators in pre-clinical research and drug discovery.

Cell-Based Format: Eliminates tedious cell lysis and protein extraction steps by enabling direct culture and analysis in 96-well plates.
Internal Normalization: Phosphorylation levels are normalized to total cellular protein within the same well, ensuring exceptional well-to-well consistency.
High Sensitivity: Accurately detects site-specific phosphorylation in adherent cell lines with a detection threshold of at least 5,000 cells.

Quantifying GSK3Α(S21) phosphorylation status to validate PI3K/Akt pathway modulation and assess the potency of upstream kinase inhibitors.

Target validation, lead optimization, and high-throughput screening for kinase inhibitors or metabolic drug candidates.

Ideal for cell-based screening of siRNA, activators, or small-molecule inhibitors to evaluate their impact on site-specific phosphorylation and downstream metabolic or oncogenic signaling cascades.

Internal performance validation using reference standards under defined assay conditions.

Glycogen synthase kinase 3 alpha (GSK3A) phosphorylated at the serine 21 (S21) residue.

Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 454 nm)

Cells

Diabetes and metabolism, oncology therapeutics, neurodegenerative diseases, cardiac hypertrophy, etc.