SigPath™ Human/Mouse GSK3Α(S21) Phosphorylation ELISA Kit

CAT#: ITS-1125-WB8
Product Type: Assay Kit
Species: Human, rat, mouse, and canine
Target: Glycogen synthase kinase 3 alpha (GSK3A) phosphorylated at the serine 21 (S21) residue.
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies GSK3α (Ser21) phosphorylation levels in cell lysates using a colorimetric ELISA method.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse GSK3Α(S21) Phosphorylation ELISA Kit provides a rapid, cell-based solution for quantifying GSK3Α phosphorylation at Serine-21 without the need for cell lysis. This high-throughput compatible system enables precise monitoring of signal transduction pathways and drug-induced effects by normalizing phosphorylated protein levels to total cellular protein. By simplifying workflows and reducing assay time, it facilitates efficient screening of kinase inhibitors and activators in pre-clinical research and drug discovery.
Features
Cell-Based Format: Eliminates tedious cell lysis and protein extraction steps by enabling direct culture and analysis in 96-well plates.
Internal Normalization: Phosphorylation levels are normalized to total cellular protein within the same well, ensuring exceptional well-to-well consistency.
High Sensitivity: Accurately detects site-specific phosphorylation in adherent cell lines with a detection threshold of at least 5,000 cells.
Process Relevance
Quantifying GSK3Α(S21) phosphorylation status to validate PI3K/Akt pathway modulation and assess the potency of upstream kinase inhibitors.
Application Stage
Target validation, lead optimization, and high-throughput screening for kinase inhibitors or metabolic drug candidates.
Applications
Ideal for cell-based screening of siRNA, activators, or small-molecule inhibitors to evaluate their impact on site-specific phosphorylation and downstream metabolic or oncogenic signaling cascades.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Glycogen synthase kinase 3 alpha (GSK3A) phosphorylated at the serine 21 (S21) residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 454 nm)
Sample Type
Cells
Research Areas
Diabetes and metabolism, oncology therapeutics, neurodegenerative diseases, cardiac hypertrophy, etc.
Size
100 tests
Data Output Type
Semi-quantitative measurement of GSK3Α(S21) phosphorylation relative to total cellular protein via fluorometric or colorimetric detection.
Assay Time
6.5 h
Species
Human, rat, mouse, and canine
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GSK3Α is a constitutively active kinase that negatively regulates glycogen synthesis and Wnt signaling. Its inhibition, triggered by Akt-mediated phosphorylation at serine 21, is a fundamental regulatory switch in glucose homeostasis.
Pathways
This kit monitors the PI3K/Akt/GSK3Α axis, where insulin or growth factors activate Akt to phosphorylate GSK3Α(S21). This inhibitory event prevents the phosphorylation of downstream substrates like glycogen synthase and β-catenin.
For Research Use Only | Not For Clinical Use
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