SigPath™ Luciferase Reporter Gene Assay Kit

CAT#: ITS-1125-YL12
Product Type: Assay Kit
Species: All Species
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies luciferase activity using a bioluminescence method.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
This SigPath™ Luciferase Reporter Gene Assay Kit offers a rapid and sensitive bioluminescent method for quantifying firefly luciferase expression in transfected cells. The system utilizes a high-activity substrate formulation to produce a stable light signal proportional to the protein concentration. This versatile tool is ideal for researchers evaluating transcriptional activity and signaling pathway dynamics, providing a streamlined workflow and high-throughput compatibility for fundamental molecular research.
Features
Delivers high-sensitivity bioluminescent detection with an optimized stable signal, facilitating rapid screening of gene regulation and transcriptional activity in high-throughput cell-based models.
Process Relevance
Quantifying reporter enzyme activity to evaluate gene regulatory mechanisms and signal transduction efficiency.
Application Stage
Functional genomics and high-throughput gene regulation screening.
Applications
Quantify firefly luciferase expression in transfected cells to analyze transcriptional regulation and signal transduction pathways in vitro.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Luminescence
Sample Type
Cell lysates
Research Areas
Gene Expression; High-Throughput Screening; Cell Biology
Size
200 tests
Data Output Type
Relative Light Units (RLU)
Assay Time
2 min
LOD
LOD: 2 fg luciferase
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Bioluminescence is a highly sensitive and versatile detection method used to monitor transcriptional regulation in live cells or lysates. The high signal-to-noise ratio of this system makes it ideal for detecting subtle changes in gene expression.
Pathways
Luciferase activity serves as a downstream reporter for gene transcription events initiated by upstream ligand-receptor interactions. It is frequently used to map intracellular signaling responses to hormones, cytokines, and drugs.
For Research Use Only | Not For Clinical Use
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