Short Description
Quantifies NFκB (p65) phosphorylation at Ser536 in cell lysates using an ELISA-based colorimetric method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ NFκB Phosphorylation Assay Kit provides a streamlined, cell-based ELISA for quantifying p65 (Ser-536) phosphorylation in cultured cells. By eliminating cell lysis and incorporating total protein normalization, this high-throughput system ensures precise measurement of NF-κB activation. It is an essential tool for screening anti-inflammatory drug candidates and studying complex signaling kinetics in pharmaceutical research.
Features
High Sensitivity & Specificity: Employs advanced ELISA-based sandwich technology to detect low-abundance phosphorylated NFκB p65 with exceptional signal-to-noise ratios.
Streamlined Homogeneous Format: Features a rapid, simplified workflow that minimizes manual handling, ensuring high reproducibility and scalability for automation.
Universal Sample Compatibility: Optimized for diverse biological matrices, including cell lysates and tissue homogenates, facilitating broad translational research applications.
Quantitative Signal Detection: Delivers precise colorimetric or fluorometric data output, allowing for rigorous kinetic modeling and comparative statistical analysis.
Process Relevance
Quantifying p65 phosphorylation to evaluate inflammatory signaling modulation and assess the potency of NFκB pathway inhibitors.
Application Stage
Target validation, high-throughput lead optimization, and pre-clinical efficacy monitoring in drug discovery pipelines.
Applications
Assessing nuclear translocation and activation across multiple cell lines, screening small molecule libraries, and investigating cytokine-induced inflammatory responses in complex primary cell cultures.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Phosphorylated NFκB (p65) subunit at the serine 536 residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 452 nm)
Research Areas
Oncology, immunology, chronic inflammatory diseases, drug discovery, cardiovascular pathology, etc.
Datasheet