T and B Cell-based ELISA Assay

A most elaborate study on epitope analysis can provide detailed information on the peptides in a protein antigen directly associated with T cell recognition and antibody binding. Using the versatile ELISA assay, Creative Biolabs offers cancer epitope analysis service by detecting the cytokines released by epitope-specific immune cells.

Enzyme-linked Immunosorbent Assay (ELISA) for Cytokines Test

Cytokines are soluble proteins of polypeptides or glycoproteins that are secreted by immune cells, including neutrophils, monocytes, macrophages, B cells, and T cells at a certain period, to modulate immune responses to stimulus. Enzyme-linked immunosorbent assay is the gold standard of immunoassays in the measurement of cytokines in plasma and cell cultures. The assay is based on antigen-antibody-specific binding and enzymatic reactions conjugated to the detection antibody.

ELISA-based Cancer Epitope Analysis Services at Creative Biolabs

The measurement of cytokines release is an effective approach to monitoring epitope-specific T-cell and B-cell responses. Now, we are proud to introduce the most popular ELISA-based method for cancer epitope analysis.

Workflow of the ELISA Assay

Work Steps of Sandwich ELISA

Protocol for sandwich ELISA. (Lin, et al., 2016) Fig.1 Protocol for sandwich ELISA.¹

Fixed capture antibody on a solid plate
Add cell supernatant
Add enzyme-labeled detection antibody
Removing unbound enzyme conjugate and added substrate
Detect signals by spectrophotometry

High-throughput ELISA Platform

Fig.2 96-well ELISA plate. (Howes, et al., 2014)

✔ 96 well plates
✔ 384 well plates
✔ 1,536-well plates

Allowing multiple samples tested in one experiment.

Fig.3 Immune cells for ELISA assay. (Creative Biolabs Original)

Cell in a single well can be scaled down to 10⁴.
A reduction in cell number by a factor of 4- to 50-fold over conventional assays.

Fig.4 A panel of antigen peptides. (Henson, et al., 2023)

Screen a panel of peptides to select immunodominant epitopes.

The List of T and B Cell Cytokine Analytics

We have a wide range of ELISA kits to ensure that you can choose the proper candidates for your research.

The List of T and B Cell Cytokine Analytics
IFNg	IL-5	GM-CSF	TNF	IL-2	IL-10	IL-4	TGFb
IL-6	IFNa	IFNb	IL-12	IL-13	IL-1b	IL-8	TNFa
Granzyme B	IL-9	IL-21	IL-22	IL-23	IL-17	IL-17A	Lymphotoxin A/TNFb
CCL3/MIP-1a	CXCL9/MIG	CXCL10/IP-10	CCL4/MIP-1b	Lymphotactin

Highlights

High throughput
Accurate and reliable data
High-specific and sensitive results
Optimized fast procedure and reduced assay time
Standard Calibration to NIBSC
Cost-effective

Related Cancer Epitope Analysis Services on Small Molecules

✔ T Cell-based Cytometric Bead Array Assay
✔ T and B Cell-based ELISPOT Assay
✔ T Cell-based High Throughput Multiplexed Assay

Creative Biolabs is skilled in employing various immunoassays for T-cell and B-cell on epitope screening. Please feel free to contact us for project quotations and get more detailed information.

References

Lin, Hunter. Lateral Flow-Based Enzyme-Linked Immunosorbent Assay as a Point-of-Care Technique to Predict Type I Reversal Reactions in Lepromatous Patients. Biology in South India. 2016: 1-5.
Howes, A. L.; et al. 3-Dimensional culture systems for anti-cancer compound profiling and high-throughput screening reveal increases in EGFR inhibitor-mediated cytotoxicity compared to monolayer culture systems. PloS one. 2014, 9(9): e108283.
Henson, S.N.; et al. PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide libraries. Nat Protoc. 2023, 18: 396-423.

For Research Use Only | Not For Clinical Use