T Cell-based Multimer/Tetramer Assay

Antigen-specific T cells existing in peripheral blood can respond to antigen infection quickly, and the mechanism is based on the T cell receptors (TCR) on the cell surface. Inspired by the unique binding interaction between TCR and peptide-MHC, Creative Biolabs offers synthesized peptide-MHC multimer/tetramer and its application in cancer epitope analysis.

Peptide-MHC Tetramer/Multimer

The exquisite specificity of T cell response to peptide/MHC is useful in tracing antigen-specific T cells and analyzing antigenic peptides. But the binding affinity of a single peptide/MHC to its cognate TCR is low and dissociates quickly. Tetramerized MHC molecular loaded with candidate peptides promoted the association of TCR with the complex. When labeling with fluorescent dyes, MHC tetramer/multimer could act as staining reagents for epitope-specific T cells, enabling the convenient detection by flow cytometer. Class I and class II tetramer reagents are produced differently and have been used in identifying CD8+ and CD4+ T cells, respectively.

Fig.1 Structure of peptide-MHC tetramer and dextramer.¹

Peptide-MHC Multimer Assay Service at Creative Biolabs

With our deep knowledge of T cell structure and function, Creative Biolabs has developed a series of peptide/MHC molecules. The tetramer and multimer produced using advanced and mature protein design and expression system show high bioactivity and natural structure.

Workflow of Tetramer-Guided Epitope Mapping (TGEM)

TGEM precisely maps antigenic epitopes of known antigens and determines the HLA restrictions.

Highlights of Our Services

• High Specificity and Reactivity
  The unspecific binding of CD8+ T cells to MHC multimer/tetramer has been diminished through sequence modification of HLA domains, which dramatically elevated the detection specificity of the T cells.
• High Quality and Efficiency
  We adopt superior material for tetramer/multimer production, ensuring the high quality and efficiency of the synthesis. With the assurance of strict quality control throughout the whole production process, our MHC multimer/tetramer demonstrates a high specificity and clear discrimination of antigenic epitopes.
• A Large Bank of MHC Alleles
  We possess more than 60 types of HLA alleles for tetramer production, which permit customized production to clients' objectives.

Equipped with advanced technologies, Creative Biolabs is devoted to offering multiple T and B cell-based assays for cancer epitope analysis. We are also experienced in developing epitope-targeted immunotherapies for cancer and autoimmune diseases. If you are interested in learning more about Creative Biolabs' services, please do not hesitate to contact us for more details.

Reference

1. Christophersen, A. Peptide-MHC class I and class II tetramers: From flow to mass cytometry. HLA. 2020, 95(3): 169-178.

For Research Use Only | Not For Clinical Use