T24 In Vitro XTT Assay (Cell Viability)

This assay is to provide T24-based In Vitro XTT Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

T24

Human

The T24 cell line was established from a transitional cell carcinoma of the bladder in a female patient in 1973. Reports in the literature indicated a wide range of tumorigenicity for T24 when injected subcutaneously in immunocompromised murine hosts.

Bladder Cancer

Oncology

In Vitro XTT Assay (Cell Viability)

T24-cell based In Vitro XTT Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) can also generate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubilization step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins