Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide TC-71-based In Vitro WST Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
TC-71
Target Cell Organism
Human
Target Cell Background
Established from the tumor of a 22-year-old man with metastatic Ewings sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site.
Related Diseases
Ewing Sarcoma
Research Area
Oncology
Assay Name
In Vitro WST Assay (Cell Viability)
Short Description
TC-71-cell based In Vitro WST Assay (Cell Viability)
Assay Description
Apart from the MTT tetrazolium, other tetrazolium compounds such as WST (reagent series) can also gener ate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubiliza tion step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins