TF-1 In Vitro Capillary Chamber Cell Migration Assay (CAT#: ITS-1122-YF1709)

TF-1

Human

TF-1 erythroblasts are isolated from bone marrow derived from a 35-year-old Asian male with severe pancytopenia in 1987. The cells provide a good system for investigating the proliferation and differentiation of myeloid progenitor cells.

Erythroleukemia

Oncology

In Vitro Capillary Chamber Cell Migration Assay

TF-1-cell based In Vitro Capillary Chamber Cell Migration Assay

Like the Boyden chamber assay, the capillary chamber migration assay also contains two separate chambers (one comprising of the cells and the other with a chemoattractant) connected with a small capillary. Cell migration and morphological responses in this assay can be observed by time-lapse microscopy. Automated real-time monitoring is also possible with this method and this assay is ideal for a rare type of cells and compounds, which are available in small quantities.

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two dif ferent processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay