Triplex Formation based DNA targeting Therapeutic Development Service

Introduction of Triplex-formation-based DNA-targeting Therapeutic Development Service

Triplex-formation-based DNA targeting is a revolutionary precision medicine approach, offering unprecedented control over genetic function. This method relies on triplex-forming oligonucleotides (TFOs), which bind specifically within the DNA major groove via Hoogsteen interactions, creating a stable triple helix. This direct DNA modulation provides unparalleled sequence specificity, allowing for precise interference with fundamental genetic processes like transcription and replication by physically blocking enzyme access or altering local DNA structure.

What We Can Offer

Customized TFO Design & Synthesis

Our service goes beyond standard oligonucleotide synthesis. We meticulously design and synthesize tailored TFOs specifically engineered to your unique target sequence. This involves incorporating advanced chemical modifications, such as 2'-O-methyl RNA, LNAs, PNAs, and various base analogues.

Comprehensive Validation

We provide robust and verifiable data through rigorous in vitro and preclinical in vivo studies. Our in vitro assays confirm triplex formation kinetics, target engagement, and functional efficacy. For preclinical in vivo studies, we assess the TFO's pharmacokinetics and pharmacodynamics, alongside initial safety profiles.

Advanced Problem-Solving Expertise

Our seasoned team excels at proactively identifying and overcoming complex challenges inherent in triplex technology. This includes mitigating pH-dependent binding of certain TFO motifs, enhancing nuclease resistance to improve in vivo half-life, preventing problematic G-quadruplex formation, and minimizing off-target interactions.

Collaborative Partnership

We believe in a true partnership approach. We work hand-in-hand with your team, fostering transparent communication and actively adapting our services to your specific research objectives and evolving project needs. This collaborative model ensures a truly customized and successful development journey, maximizing the potential of your therapeutic candidates.

Highlights

Unrivaled Expertise & Innovation

Our unparalleled expertise is backed by a proven track record in overcoming the most formidable challenges, from advanced TFO design and sophisticated chemical modifications for enhanced stability and specificity to optimizing complex delivery strategies.

Tailored Solutions & Collaborative Partnership

Our flexible framework ensures a highly efficient and personalized development process, where your insights are integrated at every stage to maximize project success and therapeutic potential.

Cutting-Edge Technology & Accelerated Timelines

Our advanced technological capabilities ensure rapid progression from initial target identification to preclinical proof-of-concept, reducing your development timelines.

Uncompromising Quality & Data Integrity

We implement rigorous, multi-tiered quality control and validation at every stage to ensure impeccable data integrity and reproducibility, providing a solid foundation for regulatory submissions and results you can trust implicitly.

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Customer Reviews

Accelerated Preclinical Data
Creative Biolabs' comprehensive workflow, from TFO design to in vivo proof-of-concept, greatly facilitated our preclinical studies. The detailed reports and robust data provided were instrumental in securing our next round of funding. Their expertise in delivery systems was particularly invaluable. - Prof. L. Mar***

Overcoming Stability Challenges
We struggled with the stability of our TFOs in cellular environments. Creative Biolabs' team introduced novel backbone modifications that dramatically increased nuclease resistance, allowing our therapeutic to maintain its integrity and function, and a critical advantage over previous attempts. - Dr. C. Joh***

FAQs

What DNA sequences can TFOs target?

TFOs primarily target homopurine: homopyrimidine DNA. Creative Biolabs' advanced chemical modifications, including the strategic incorporation of base analogues like 2-aminopyridine, enable effective targeting of sequences containing pyrimidine interruptions, significantly expanding the range of accessible genomic sites. This allows for broader applicability across various therapeutic targets.

How does Creative Biolabs ensure TFO stability and delivery?

We use backbone modifications (e.g., 2'-O-methyl RNA, PNAs, LNAs) for nuclease resistance and stability. For delivery, we optimize cutting-edge strategies including the encapsulation of TFOs within LNPs for systemic delivery, or the conjugation of TFOs to cell-penetrating peptides or specific targeting ligands to facilitate uptake into desired cell types and precise nuclear localization.

Do TFOs interfere with other DNA processes?

Yes, TFOs block the DNA major groove, preventing binding of proteins like transcription factors or polymerases. This direct binding prevents the access of crucial cellular machinery, including transcription factors, RNA polymerases, and even certain DNA repair enzymes. By forming a stable triple helix, TFOs can precisely inhibit gene transcription, interfere with DNA replication, or modulate DNA repair pathways.

Related Services

In Vivo PK Service

We analyze what the body does to the TFO. This involves a meticulous assessment of the TFO's entire journey within a living system, including its absorption, distribution, metabolism, and excretion.

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In Vivo PD

We evaluate what the TFO does to the body. Using relevant animal models, such as murine models for oncology, we measure their on-target activity and their duration of effect.