Vesitra™ Dog MDR1 Stable MDCK Cell Line

CAT#: ITS-0326-HM32
Product Type: Transporter Cell Line
Organism: Canine
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Metabolism; Absorption; Excretion
Short Description
Canine MDR1 stable expression MDCK model, vital for interpreting preclinical dog DMPK data and assessing breed-specific toxicity risks.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Metabolism; Absorption; Excretion
Description
Dogs are a primary non-rodent species for preclinical safety evaluation. The Vesitra™ Dog MDR1 cell line captures the unique substrate affinities of canine P-glycoprotein, enabling researchers to predict canine oral absorption and brain penetration, and to assess potential neurotoxic sensitivities prior to in vivo studies.
Features
1. Accurately reflects the distinct transport kinetics and substrate specificities of canine P-gp. 2. Forms tight, polarized cell layers enabling definitive efflux ratio calculations. 3. Validated specifically to support IND-enabling toxicology species selection.
Applications
Predicting canine oral bioavailability and BBB permeability during preclinical development; Veterinary pharmacology research targeting breed-specific MDR1 vulnerabilities.
Parental Cell Line
Madin-Darby Canine Kidney
Transfection
Stable Transfection
Transporter
MDR1
Gene
ABCB1
Typical Probe Substrate
Digoxin
Reference Inhibitor
Verapamil
Organism
Canine
Strain Name
MDCK
Tissue
Kidney
Background
Dog MDR1 (ABCB1) expresses the canine ortholog of P-gp. Beagles are a primary non-rodent species used in pivotal preclinical toxicology studies. Utilizing this canine-specific in vitro model is vital for predicting potential DDI liabilities and neurotoxicity in dogs, ensuring the validity and species relevance of the preclinical safety data package.
Morphology
Epithelial
Unit Size
2×10⁶ / vial lyophilized cells
Culture Properties
Adherent
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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