Vesitra™ Human MRP3 Membrane Vesicles

CAT#: ITS-0326-HM38
Product Type: Transporter Vesicles
Brand: Vesitra™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
A dedicated vesicle model for investigating basolateral hepatic efflux and enterohepatic circulation dynamics.
Description
Vesitra™ Human MRP3 Membrane Vesicles highlight the compensatory basolateral efflux pathways in the liver. When biliary excretion is blocked, MRP3 serves as a vital escape valve, pumping bile acids and drug conjugates back into the blood. This model helps researchers accurately map hepatic disposition and alternative clearance routes.
Brand
Vesitra™
Development Stage
ADME
ADME Research Domain
Excretion
Features
1. Exceptional sensitivity for detecting the transport of bile acid conjugates and glucuronides. 2. Clean background without overlapping interference from canalicular transporters. 3. Highly stable vesicle integrity during extended ATP-driven assays.
Applications
Studying compensatory hepatic efflux mechanisms during cholestasis; Evaluating the reverse transport of drug glucuronides back into the systemic circulation.
Species
Human
Expression System
Baculovirus-infected Sf9 insect cells
Physical State
Ready-to-use Suspension
Transporter
MRP3
Gene
ABCC3
Probe Substrate
Estradiol-17β-D-glucuronide
Reference Inhibitor
MK-571
Protein Concentration
5 mg/mL
Total Protein
2.5 mg per vial
Volume
0.5 mL; 1 mL
Vesicle Type
Inside-out ABC Transporter Membrane Vesicles
Background
MRP3 (ABCC3) is localized on the basolateral membrane of hepatocytes and enterocytes. Its expression is strongly upregulated during cholestatic conditions, acting as a crucial overflow pump that redirects toxic bile acids and drug metabolites from the liver back into the bloodstream for subsequent renal clearance.
Shipping
Dry ice
Storage
Store immediately at -80°C upon receipt. Do not store in liquid nitrogen or standard -20°C freezers.
Handling Notes
1. Thaw the vial quickly by gently agitating it in a 37°C water bath (usually takes 1-2 minutes).
2. Immediately place the vial on ice once thawed.
3. Avoid repeated freeze-thaw cycles. If aliquoting is necessary, do so rapidly on ice immediately after the initial thaw, and snap-freeze the aliquots back at -80°C.
Background Control
It is highly recommended to perform assays alongside Mock/Control Vesicles (derived from cells infected with an empty vector) to subtract non-specific background binding.
Batch-to-Batch Consistency
A lot-specific Certificate of Analysis (CoA) is provided with each shipment, documenting the exact ATP-dependent uptake rates (e.g., pmol/mg protein/min).
For Research Use Only | Not For Clinical Use
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