The emergence of breakthrough technologies in oncology imaging technology - allows scientists to provide gene-targeting lineage tracing technology making it possible to monitor tumor progress. Creative Biolabs offers the best guarantee in tumor imaging and has successfully developed a series of FLP/FRT site-specific recombination system-mediated lineage tracing systems for various types of cancer therapy.

Introduction to Yeast FLP/FRT Site-specific Recombination System

The FLP/FRT system is a typical two-component site-specific recombination system. The full length of the FLP recombinase gene is 1,272 bp, encoding a 48 kD polypeptide monomer protein of 423 amino acid residues. There are two types of mutant recognition sites in the FLP/FRT system, one is an inverted repeat mutation site, and the other is a spacer mutation site. Currently, spacer region mutant FRT sites have been widely used in genetic engineering research in higher eukaryotic model organisms such as drosophila and mice.

As a result, Creative Biolabs has established a yeast FLP/FRT site-specific recombination system, which can achieve precise regulation of tumor-related gene targets in time and space, effectively promoting the rapid development of functional gene research in tumor treatment. Moreover, we have also developed a novel tumor cell lineage tracing technology based on a regular FLP/FRT system to reveal the proliferation, differentiation, and migration processes of tumor cells and their offspring through various labeling methods.

This technique enables the tracking of the growth and spread of thousands of human metastatic cancer cells over six months. In addition, the data obtained from our system can provide information on many other difficult-to-observe aspects of cancer cell biology, like genetic mutations, microenvironmental adaptation, and drug resistance.

Table 1 Available FLP recognition sites

Main Services

• Gene-modified mouse model research and development services
• Mouse and rat model phenotypic analysis services
• Tumor evolutionary lineage dynamic tracking services
• Tumor plasticity and path analysis services

Our Tumor Lineage Tracing Imaging Platform

To date, we can design and build a range of marker tracer assays, and our researchers will choose the appropriate tracer method based on the customer's research purpose, the cell situation, or animal experiment type, and explore the advantages of multi-modal marker tracer modes to achieve the best data. Besides, our labs are equipped with a full set of in-vivo tracer imaging equipment to provide the most advanced optical imaging, nuclear magnetic resonance imaging, and nuclide imaging services, thus achieving continuous real-time tracer observation of labeled tumor cells.

1. Advantages of optical imaging: easy operation, high sensitivity, non-invasive, non-toxic, non-radioactive, relatively low cost. Optical imaging can be divided into two categories: biological self-luminescence imaging and excited fluorescence imaging.
2. Advantages of MRI: high spatial resolution, strong sensitivity, mostly used in the field of cell therapy.
3. Advantages of nuclide imaging technology: high sensitivity and specificity, imaging can be performed at any wavelength, and no excitation light is required.

Creative Biolabs has extensive experience in the field of tumor lineage tracing and can provide you with quality services and technology. Up to now, we have offered a broad range of yeast FLP/FRT site-specific recombination system solutions at a reasonable cost and with a quick turnaround time. Our highly experienced scientists are ready to tackle difficult projects to meet any requirement. If you are interested in our services, please feel free to contact us for closer communication to learn how we can be involved in your project. Separate services or integrated end-to-end solutions are all welcomed.

For Research Use Only | Not For Clinical Use