As allowing the isolation of native antibodies with the preservation of the natural VH and VL pairing, Native™ Antibody Discovery has become attractive to sample naïve and antigen-experienced antibody repertoires generated in vivo over the last decade. Laser-capture microdissection (LCM) is an advanced and representative technique to isolate individual cells or cell compartments from most solid tissue samples of diverse species. Creative Biolabs has developed a novel LCM antibody discovery system to provide a rapid and reliable approach for high-quality antibodies generation.
How Does Laser-capture Microdissection Work?
As a new technology, LCM system is a commonly applied method of choice, whenever single cells need to be isolated from solid samples (e.g., tissue, biopsies) typically provided fixed in formalin, embedded in paraffin, or cryo-fixed. Based on optical microscopes coupled with a coaxial cutting laser and computer-assisted control, LCM system can successfully capture and extract cells in all tissue cells on the cap to procure pure populations of targeted cells from specific microscopic regions of tissue sections, which can be used for subsequent single-cell RT-PCR without damaging the enzymatic function. LCM is compatible with a variety of tissue types, cellular staining methods and tissue preservation protocols that allow selective procurement of fresh or archival specimens with the precision of 3-5 mm.
Fig.1 Schematic of Laser-capture microdissection technology.
Features of Laser-capture Microdissection
Customization Outputs of LCM-based Antibody Discovery System
1. Laser-capture microdissection can be a contact- and contamination-free method for isolating specific single cells from a wide variety of tissue samples depending on your requirements.
2. The use of LCM in combination with antibody discovery offers a rapid, versatile and rational method for the generation of high affinity and discriminative human monoclonal antibodies.
3. Our system establishes paired data library capturing native VH-VL chains, scalable to the consumer needs.
4. Our one-package service includes identifying and selecting cells, isolating and purifying RNA, single-cell cDNA synthesis, cDNA fragments amplifications and analysis, expression systems or expression-vectors (IgH, IgL) selection, expression and purification of your desired antibodies and even affinity purification of antibodies.
Emerging as one of the most user-friendly, reliable, and consistent single cell collection systems, LCM systems are perfect tools to optimize Native™ Antibody Discovery workflows under visual control for high-efficient antibody generation. Creative Biolabs can provide LCM platforms allowing for dissection of living tissue, enabling the extraction of live cells to satisfy our customers’ particular needs.