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LGR6 Membrane Protein Introduction

Introduction of LGR6

LGR6, also known as GPCR or VTS20631, is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily, encoded by LGR6 gene. It is highly homologous to LGR4 and LGR5 with 50% identity between each other at the amino acid level. The LGR4–6 is characterized by a large N-terminal extracellular domain (ECD) containing 17 leucine-rich repeats which are flanked by cysteine-rich sequences at both the N- and C-termini. And the 17 leucine-rich repeats are very important for the interaction with ligands.

Basic Information of LGR6
Protein Name Leucine-rich repeat-containing G-protein coupled receptor 6
Gene Name LGR6
Aliases GPCR, VTS20631
Organism Homo sapiens (Human)
UniProt ID Q9HBX8
Transmembrane Times 7
Length (aa) 967
Sequence MPSPPGLRALWLCAALCASRRAGGAPQPGPGPTACPAPCHCQEDGIMLSADCSELGLSAVPGDLDPLTAYLDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLYSLKILMLQNNQLGGIPAEALWELPSLQSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNRISHIPDYAFQNLTSLVVLHLHNNRIQHLGTHSFEGLHNLETLDLNYNKLQEFPVAIRTLGRLQELGFHNNNIKAIPEKAFMGNPLLQTIHFYDNPIQFVGRSAFQYLPKLHTLSLNGAMDIQEFPDLKGTTSLEILTLTRAGIRLLPSGMCQQLPRLRVLELSHNQIEELPSLHRCQKLEEIGLQHNRIWEIGADTFSQLSSLQALDLSWNAIRSIHPEAFSTLHSLVKLDLTDNQLTTLPLAGLGGLMHLKLKGNLALSQAFSKDSFPKLRILEVPYAYQCCPYGMCASFFKASGQWEAEDLHLDDEESSKRPLGLLARQAENHYDQDLDELQLEMEDSKPHPSVQCSPTPGPFKPCEYLFESWGIRLAVWAIVLLSVLCNGLVLLTVFAGGPVPLPPVKFVVGAIAGANTLTGISCGLLASVDALTFGQFSEYGARWETGLGCRATGFLAVLGSEASVLLLTLAAVQCSVSVSCVRAYGKSPSLGSVRAGVLGCLALAGLAAALPLASVGEYGASPLCLPYAPPEGQPAALGFTVALVMMNSFCFLVVAGAYIKLYCDLPRGDFEAVWDCAMVRHVAWLIFADGLLYCPVAFLSFASMLGLFPVTPEAVKSVLLVVLPLPACLNPLLYLLFNPHFRDDLRRLRPRAGDSGPLAYAAAGELEKSSCDSTQALVAFSDVDLILEASEAGRPPGLETYGFPSVTLISCQQPGAPRLEGSHCVEPEGNHFGNPQPSMDGELLLRAEGSTPAGGGLSGGGGFQPSGLAFASHV

Function of LGR6 Membrane Protein

It has been shown that LGR6 can recover the effect of R-spondins on Wnt/β-catenin signaling when LGR4 is knocked down in HEK293T cells. Hence, LGR6 functions similarly as LGR4 and LGR5. It can also interact with R-spondins, a group of four secreted proteins (RSPO1–4) and positively promote the activation of Wnt/β-catenin signaling. LGR6 can regulate multiple biological processes via mediating Wnt/β-catenin signaling, including cell proliferation, differentiation, and apoptosis. Studies indicate that LGR6 can be regarded as an epithelial stem cell marker that can give rise to all cell lineages of the skin, including those of the hair follicle, sebaceous gland, and interfollicular dermis. Moreover, LGR6 may play a role in the pathological process of many tumors, such as colon cancer, ovary cancer, pancreas cancer. The results have shown that overexpression of wild-type LGR6 in HeLa cell could lead to an increase of cell migration and LGR6 is hypermethylated in about 50% of colon cancer.

RSPO augmentation of Wnt signaling.Fig.1 RSPO augmentation of Wnt signaling. (Schindler, 2018)

Application of LGR6 Membrane Protein in Literature

  1. Ke L., et al. LGR6 promotes the progression of gastric cancer through PI3K/AKT/mTOR pathway. OncoTargets and Therapy. 2018, 11:3025-3033. PubMed ID: 29872314

    The study indicates that LGR6 acts as an antiapoptosis protein which promotes the progression of gastric cancer through PI3K/AKT/mTOR pathway.

  2. Wang F., et al. Downregulation of Lgr6 inhibits proliferation and invasion and increases apoptosis in human colorectal cancer. International Journal of Molecular Medicine. 2018, 42(1):625-632. PubMed ID: 29693156

    The study reports that LGR6 promotes proliferation and invasion of colorectal cancer, suggesting that LGR6 may act as a potential diagnostic and prognostic biomarker for colorectal cancer.

  3. Cui Y., et al. Down-regulation of LGR6 promotes bone fracture recovery using bone marrow stromal cells. Biomedicine & Pharmacotherapy. 2018, 99:629-637. PubMed ID: 29625528

    The authors propose that the inhibition of LGR6 can promote osteogenic differentiation of bone marrow stromal cells (BMSCs) and bone healing.

  4. Schindler A.J., et al. LGR5 and LGR6 in stem cell biology and ovarian cancer. Oncotarget. 2018, 9(1):1346-1355. PubMed ID: 29416699

    In this review, authors summarize current data about LGR5 and LGR6 in the ovarian surface epithelium (OSE) and fallopian tube epithelium (FTE). LGR5 and LGR6 are required in the process of recovery from injury and they are the key determinants of stem cell number. Besides, LGR5 and LGR6 are involved in the pathological process of cancer, suggesting LGR5 and LGR6 can act as the therapeutic targets for cancer treatment.

  5. Huang P.Y., et al. Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma. Nature Genetics. 2017, 49(11):1624-1632. PubMed ID: 28945253

    The findings show that LGR6 expression is enriched in cells with stem cell characteristics, and LGR6 downregulation in vivo causes increased epidermal proliferation, suggesting LGR6 is a stem cell marker in mouse skin squamous cell carcinoma.

LGR6 Preparation Options

Based on the versatile Magic™ membrane protein production platform, Creative Biolabs can provide many flexible options for soluble and functional target protein, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-LGR6 antibody development services.


As a forward-looking research institute as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.

Reference

  1. Huang P Y, et al. (2018). Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma. Nature Genetics. 49(11), 1624-32.

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