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ligation of Fab gene into phagemid and transformation

  1. The product of over-lapping PCR and Fab expression cassette are cut with SfiI and ligated into pCDisplay-4TM.
  2. Desalt the ligation mix and electro-transform into TG1 competent cells of high efficiency made with a novel method developed by CBL. The detailed protocol should not be disclosed here.

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