This study aims to investigate the impact of the ratio of DC-Chol and DOPE in cationic liposomes on transfection efficiency. Researchers first prepared two groups of DC-Chol:DOPE liposomes (with and without PEGylation), with DC-Chol to DOPE ratios of 3:1, 2:1, 1:1, 1:2, and 1:3 in each. Subsequently, plasmid DNA was added to the liposome solution to form liposome/DNA complexes, known as DNA lipoplexes. The results (figure a/b) demonstrate that PEGylation can lessen the toxicity of DC-Chol:DOPE liposomes to SKOV-3 cells, and that lower proportions of cationic lipid (DC-Chol) result in weaker cellular toxicity. Following in vitro transfection tests, the transfection efficiency of different PEGylated liposomes (1:1, 1:2, and 1:3 DC-Chol/DOPE) in the presence or absence of serum was ranked from greatest to lowest as follows: 1:1, 1:2, 1:3 (figure c). This work reveals that PEGylated DC-Chol:DOPE liposomes may be used as a gene delivery platform with low cellular toxicity, with the highest transfection efficiency attained when the DC-Chol:DOPE ratio is set to 1:1.
Sun M, et al. "Physicochemical Factors That Influence the Biocompatibility of Cationic Liposomes and Their Ability to Deliver DNA to the Nuclei of Ovarian Cancer SK-OV-3 Cells." Materials (Basel). 14.2 (2021):416. Under Open Access license CC BY 4.0, the image is a composite of figure 1 and figure 7.
For Research Use Only. Not For Clinical Use 