DDAB:DOPE Liposomes (CAT#: LDLY-0123-LY184)

Description
Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DDAB
DOPE
Applications
Cancer research; Target delivery
Format
Liquid
Hydration Solution
Deionized RNAse-free Water
External Solution
Deionized RNAse-free Water
Storage
2°C - 8°C in dark (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Download
DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. What are DDAB:DOPE liposomes and what is their primary use in research?

    DDAB:DOPE liposomes are cationic liposomes formed by the combination of DDAB (Dimethyldioctadecylammonium bromide) and DOPE (Dioleoylphosphatidylethanolamine). These liposomes are primarily used for the delivery of genetic materials such as DNA, RNA, siRNA, and other nucleic acids into cells. Their positive charge aids in the efficient encapsulation and cellular uptake of negatively charged genetic materials.

  2. How do DDAB:DOPE liposomes enhance gene delivery compared to other delivery systems?

    The cationic nature of DDAB:DOPE liposomes facilitates the formation of complexes with negatively charged nucleic acids, improving encapsulation efficiency and protection of the genetic material from degradation. Additionally, the presence of DOPE, a fusogenic lipid, enhances the fusion of liposomes with cell membranes, promoting efficient intracellular delivery of the encapsulated nucleic acids.

  3. What are the key characteristics of DDAB:DOPE liposomes that researchers should consider?

    Key characteristics include the size distribution, zeta potential, and encapsulation efficiency of the liposomes. The mean particle size is typically 100 nm, which is optimal for cellular uptake. The zeta potential indicates the surface charge, crucial for forming stable complexes with genetic material. Encapsulation efficiency determines the amount of nucleic acid that can be successfully loaded into the liposomes.

  4. Can DDAB:DOPE liposomes be used for in vivo experiments?

    While primarily designed for in vitro research applications, DDAB:DOPE liposomes can also be used for in vivo studies. Researchers must carefully consider factors such as biodistribution, clearance rates, and potential immunogenicity. It is recommended to perform thorough preliminary studies to assess the feasibility and safety of using these liposomes in vivo.

  5. How can the efficiency of gene delivery using DDAB:DOPE liposomes be optimized?

    Efficiency can be optimized by adjusting the ratio of liposomes to nucleic acids, incubation time, and the conditions of the cell culture medium. It is also important to fine-tune the liposome formulation by altering the lipid composition or incorporating additional components that can enhance cellular uptake and endosomal escape. Empirical testing is essential to determine the most effective conditions for each specific application.

DDAB:DOPE (50:50) Liposomes-fig1


In vitro protein expression after transfection with mRNA lipoplexes.

The study focuses on preparing cationic liposome/mRNA complexes (mRNA lipoplexes) for mRNA delivery through the modified ethanol injection (MEI) method. The researchers evaluated the transfection efficiency of 18 different mRNA lipoplexes prepared using 8 cationic lipids (DOTAP, DC-1-14, DC-1-16, DDAB, DC-6-14, TC-1-12) and 3 helper lipids (DOPE, DOPC, Cholesterol). In this study, the expression of luciferase and EGFP in hela cells after transfection with mRNA lipoplexes was studied. The data showed that DOPE, as a helper lipid, can induce a higher level of transfection efficiency. The transfection efficiency of the six lipoplexes ranked from high to low as follows: TC-1-12/DOPE, DC-1-16/DOPE, DC-1-14/DOPE, DOTAP/DOPE, DC-6-14/DOPE, DDAB/DOPE. This study demonstrates the effectiveness of TC-1-12/DOPE-based lipoplexes as mRNA delivery carriers, highlighting the significance of lipid composition in enhancing gene delivery.

Tang, Min, et al. "Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method." Pharmaceutics. 15.4 (2023): 1141. Under Open Access license CC BY 4.0, without modification.

  • Unmatched Performance in RNA Delivery
    Utilizing DDAB:DOPE liposomes from Creative Biolabs transformed our RNA-based therapies research. The superior encapsulation and delivery rates are unmatched, underscoring their pivotal role in advancing RNA therapeutics.
  • Benchmark for Vaccine Formulation Studies
    In the realm of vaccine formulation, Creative Biolabs' DDAB:DOPE liposomes set a high benchmark. Their stability and biocompatibility are exceptional, providing reliable results in our most critical studies.
  • Elevating Drug Development Processes
    DDAB:DOPE liposomes have elevated our drug development processes to new heights. Their formulation ensures targeted delivery and release, crucial for the success of next-generation pharmaceuticals.
  • Gold Standard in Liposome Research
    Among peers, Creative Biolabs' DDAB:DOPE liposomes are the gold standard. Their consistent performance and quality in drug delivery applications make them a preferred choice for researchers worldwide.
  • Pioneering Solutions for Nucleic Acid Delivery
    The versatility of DDAB:DOPE liposomes in nucleic acid delivery is unparalleled. Their use has led to groundbreaking findings in gene therapy research, making them an indispensable tool for innovative studies.

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For Research Use Only. Not For Clinical Use

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