DDAB:DOPE liposomes are cationic liposomes formed by the combination of DDAB (Dimethyldioctadecylammonium bromide) and DOPE (Dioleoylphosphatidylethanolamine). These liposomes are primarily used for the delivery of genetic materials such as DNA, RNA, siRNA, and other nucleic acids into cells. Their positive charge aids in the efficient encapsulation and cellular uptake of negatively charged genetic materials.
The cationic nature of DDAB:DOPE liposomes facilitates the formation of complexes with negatively charged nucleic acids, improving encapsulation efficiency and protection of the genetic material from degradation. Additionally, the presence of DOPE, a fusogenic lipid, enhances the fusion of liposomes with cell membranes, promoting efficient intracellular delivery of the encapsulated nucleic acids.
Key characteristics include the size distribution, zeta potential, and encapsulation efficiency of the liposomes. The mean particle size is typically 100 nm, which is optimal for cellular uptake. The zeta potential indicates the surface charge, crucial for forming stable complexes with genetic material. Encapsulation efficiency determines the amount of nucleic acid that can be successfully loaded into the liposomes.
While primarily designed for in vitro research applications, DDAB:DOPE liposomes can also be used for in vivo studies. Researchers must carefully consider factors such as biodistribution, clearance rates, and potential immunogenicity. It is recommended to perform thorough preliminary studies to assess the feasibility and safety of using these liposomes in vivo.
Efficiency can be optimized by adjusting the ratio of liposomes to nucleic acids, incubation time, and the conditions of the cell culture medium. It is also important to fine-tune the liposome formulation by altering the lipid composition or incorporating additional components that can enhance cellular uptake and endosomal escape. Empirical testing is essential to determine the most effective conditions for each specific application.
In vitro protein expression after transfection with mRNA lipoplexes.
The study focuses on preparing cationic liposome/mRNA complexes (mRNA lipoplexes) for mRNA delivery through the modified ethanol injection (MEI) method. The researchers evaluated the transfection efficiency of 18 different mRNA lipoplexes prepared using 8 cationic lipids (DOTAP, DC-1-14, DC-1-16, DDAB, DC-6-14, TC-1-12) and 3 helper lipids (DOPE, DOPC, Cholesterol). In this study, the expression of luciferase and EGFP in hela cells after transfection with mRNA lipoplexes was studied. The data showed that DOPE, as a helper lipid, can induce a higher level of transfection efficiency. The transfection efficiency of the six lipoplexes ranked from high to low as follows: TC-1-12/DOPE, DC-1-16/DOPE, DC-1-14/DOPE, DOTAP/DOPE, DC-6-14/DOPE, DDAB/DOPE. This study demonstrates the effectiveness of TC-1-12/DOPE-based lipoplexes as mRNA delivery carriers, highlighting the significance of lipid composition in enhancing gene delivery.
Tang, Min, et al. "Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method." Pharmaceutics. 15.4 (2023): 1141. Under Open Access license CC BY 4.0, without modification.
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