DDAB liposomes find applications in various research areas including drug delivery, gene therapy, vaccine development, and nanomedicine. Researchers utilize these versatile lipid-based carriers to deliver therapeutic agents or genetic materials with improved specificity and efficacy, contributing to advancements in biomedical science.
The optimal concentration of DDAB liposomes depends on the specific experimental setup and the intended application. Researchers typically conduct dose-response studies to determine the appropriate concentration range that achieves desired effects while minimizing potential cytotoxicity.
Several techniques can be employed to characterize DDAB liposomes, including dynamic light scattering (DLS) for size measurement and zeta potential analysis for surface charge determination. Additionally, stability studies involving storage conditions and serum stability assays can provide insights into liposomal behavior over time.
Various methods exist for loading therapeutic cargoes into DDAB liposomes, including passive encapsulation during liposome formation, active loading techniques such as pH gradient or transmembrane ammonium sulfate gradients, and post-insertion methods where preformed liposomes are incubated with the payload.
Optimization of DDAB liposome formulation involves adjusting parameters such as lipid composition, drug-to-lipid ratio, and preparation methods to achieve desired characteristics such as size, stability, and drug release kinetics. Systematic screening and characterization are essential for identifying the optimal formulation.
Cellular uptake of LPs-6C and CLPs-6C by hCMEC/D3 cells.
This research evaluated whether cationic liposomes (CLPs) with DDAB and Tween 80 penetrated the blood-brain barrier (BBB). The BBB may prevent certain substances from entering the brain from the bloodstream, protecting the brain but also restricting treatments for brain diseases. Tween 80 may establish a coating on the surface of liposomes, giving them "stealth" properties that increase their half-life. Cationic liposomes prepared with DDAB can penetrate the BBB via electrostatic interactions with negatively charged cell membranes, allowing for efficient drug delivery to the brain. Researchers prepared cationic liposomes known as CLPs-6C, which were loaded with the fluorescent probe 6-Coumarin (6C). The blood-brain barrier permeability of CLPs-6C has been evaluated by measuring liposome uptake by hCMEC/D3 cells. The results showed that cell uptake rates for LPs-6C without DDAB and CLPs-6C with DDAB were 6.4% and 14%, respectively. This suggests that the presence of DDAB enhances cellular uptake, pointing towards a promising drug delivery method using DDAB-based liposomes for crossing the BBB.
Piazzini, Vieri, et al. "Stealth and cationic nanoliposomes as drug delivery systems to increase andrographolide BBB permeability." Pharmaceutics. 10.3 (2018): 128. Under Open Access license CC BY 4.0, without modification.
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