DDAB Liposomes (CAT#: LDLY-0123-LY182)

Description
Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DDAB
Applications
Cancer research; Target delivery
Format
Liquid
Hydration Solution
Deionized RNAse-free Water
External Solution
Deionized RNAse-free Water
Storage
2°C - 8°C in dark (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Download
DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. What are the potential research applications of DDAB liposomes?

    DDAB liposomes find applications in various research areas including drug delivery, gene therapy, vaccine development, and nanomedicine. Researchers utilize these versatile lipid-based carriers to deliver therapeutic agents or genetic materials with improved specificity and efficacy, contributing to advancements in biomedical science.

  2. What concentration of DDAB liposomes is suitable for my experiments?

    The optimal concentration of DDAB liposomes depends on the specific experimental setup and the intended application. Researchers typically conduct dose-response studies to determine the appropriate concentration range that achieves desired effects while minimizing potential cytotoxicity.

  3. How can I characterize the size and stability of DDAB liposomes?

    Several techniques can be employed to characterize DDAB liposomes, including dynamic light scattering (DLS) for size measurement and zeta potential analysis for surface charge determination. Additionally, stability studies involving storage conditions and serum stability assays can provide insights into liposomal behavior over time.

  4. What methods can be used for loading drugs or nucleic acids into DDAB liposomes?

    Various methods exist for loading therapeutic cargoes into DDAB liposomes, including passive encapsulation during liposome formation, active loading techniques such as pH gradient or transmembrane ammonium sulfate gradients, and post-insertion methods where preformed liposomes are incubated with the payload.

  5. How do I optimize the formulation of DDAB liposomes for my specific application?

    Optimization of DDAB liposome formulation involves adjusting parameters such as lipid composition, drug-to-lipid ratio, and preparation methods to achieve desired characteristics such as size, stability, and drug release kinetics. Systematic screening and characterization are essential for identifying the optimal formulation.

DDAB Liposomes-fig1


Cellular uptake of LPs-6C and CLPs-6C by hCMEC/D3 cells.

This research evaluated whether cationic liposomes (CLPs) with DDAB and Tween 80 penetrated the blood-brain barrier (BBB). The BBB may prevent certain substances from entering the brain from the bloodstream, protecting the brain but also restricting treatments for brain diseases. Tween 80 may establish a coating on the surface of liposomes, giving them "stealth" properties that increase their half-life. Cationic liposomes prepared with DDAB can penetrate the BBB via electrostatic interactions with negatively charged cell membranes, allowing for efficient drug delivery to the brain. Researchers prepared cationic liposomes known as CLPs-6C, which were loaded with the fluorescent probe 6-Coumarin (6C). The blood-brain barrier permeability of CLPs-6C has been evaluated by measuring liposome uptake by hCMEC/D3 cells. The results showed that cell uptake rates for LPs-6C without DDAB and CLPs-6C with DDAB were 6.4% and 14%, respectively. This suggests that the presence of DDAB enhances cellular uptake, pointing towards a promising drug delivery method using DDAB-based liposomes for crossing the BBB.

Piazzini, Vieri, et al. "Stealth and cationic nanoliposomes as drug delivery systems to increase andrographolide BBB permeability." Pharmaceutics. 10.3 (2018): 128. Under Open Access license CC BY 4.0, without modification.

  • Enhanced Drug Delivery Efficiency
    The DDAB liposomes from Creative Biolabs outshine competitors with superior drug encapsulation and release properties. Their formulation significantly boosts drug delivery, aiding our research in targeted therapy development.
  • Exceptional Stability and Compatibility
    Creative Biolabs' DDAB liposomes exhibit remarkable stability and compatibility, offering a reliable platform for our drug development studies. Their consistency and efficacy surpass other options in the market.
  • Optimal Particle Size and Homogeneity
    Using Creative Biolabs' DDAB liposomes has been a game-changer in our research. The precise control over particle size and homogeneity enhances drug loading efficiency, providing unparalleled results in our experiments.
  • Outstanding Cellular Uptake
    We are impressed by the exceptional cellular uptake achieved with Creative Biolabs' DDAB liposomes. Their advanced formulation facilitates efficient intracellular delivery, surpassing competitors in our drug delivery studies.
  • Superior Research Support
    Creative Biolabs' DDAB liposomes have significantly advanced our drug development efforts. Their dedicated support team ensures seamless integration into our research protocols, making them our preferred choice for lipid-based drug delivery systems.

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For Research Use Only. Not For Clinical Use

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