DOTAP:Chol Liposomes (CAT#: LDLY-0123-LY172)

Description
Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DOTAP
Chol
Applications
Cancer research; Target delivery
Format
Liquid
Hydration Solution
Deionized RNAse-free Water
External Solution
Deionized RNAse-free Water
Storage
2°C - 8°C (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Download
DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. How should DOTAP:Chol liposomes be stored for optimal performance?

    It's crucial to store these liposomes at 4°C in the dark to maintain their stability. They should not be frozen as freezing can disrupt the liposomal structure, potentially affecting their performance.

  2. What are the best practices for preparing DOTAP:Chol liposomes for experimental use?

    When preparing these liposomes for experiments, ensure that they are thoroughly mixed and homogenized. The method of adding the therapeutic agent (e.g., nucleic acids or drugs) should be carefully chosen based on the type of agent and the desired encapsulation efficiency.

  3. Can DOTAP:Chol liposomes be combined with other substances?

    Yes, these liposomes can be used in conjunction with other substances. However, it's important to assess the compatibility of these substances with the liposomes to ensure that they do not destabilize the liposomal structure or interfere with the encapsulation and release of the therapeutic agent.

  4. What factors should be considered when using these liposomes for transfection?

    For transfection applications, consider the lipid-to-DNA ratio, the health and type of cells being transfected, and the incubation conditions. Optimizing these parameters is crucial for maximizing transfection efficiency.

  5. How is the success of drug delivery using DOTAP:Chol liposomes evaluated?

    The success can be evaluated by assessing the therapeutic response, which may include measuring the levels of delivered drug or gene expression in the target cells. Techniques such as fluorescence microscopy, PCR, and cell viability assays are commonly used.

DOTAP:Chol (50:50) Liposomes-fig1


Antigen uptake by CD11c+ DCs in NALTs following intranasal administration of Alexa Fluor-labelled OVA plus the cationic liposomes.

The study focused on the potent mucosal adjuvant effect of DOTAP/chol liposomes on intranasal immunization in mice. Vaccines administered through the mucosal route have the potential to be an effective strategy for combating infectious diseases, as mucosal surfaces serve as the primary entry point for most pathogens. For the intranasal administration of ovalbumin (OVA), researchers prepared cationic liposomes with DOTAP and cholesterol. They used flow cytometry to analyze OVA (Alexa Fluor 488-labeled) uptake by dendritic cells (DCs) in nasal-associated lymphoid tissues (NALTs) following intranasal administration of OVA-loaded liposomes or OVA alone. This makes it easier to determine if cationic liposomes may trigger antigen-specific humoral immune responses in the systemic and mucosal tissues. The administration of Alexa Fluor 488-OVA with DOTAP/chol liposomes led to a greater uptake of Alexa Fluor 488-OVA in DCs of NALTs compared to administration of Alexa Fluor 488-OVA alone. This indicates that DOTAP/chol liposomes have the potential to act as mucosal adjuvants and can enhance OVA transport to NALTs.

Tada, Rui, et al. "Intranasal immunization with DOTAP cationic liposomes combined with DC-cholesterol induces potent antigen-specific mucosal and systemic immune responses in mice." PLoS One 10.10 (2015): e0139785. Under Open Access license CC BY 4.0, without modification.

  • Superior Encapsulation Efficiency
    Creative Biolabs' DOTAP:Chol liposomes showcase remarkable encapsulation efficiency, ensuring optimal drug delivery in pharmaceutical research, outshining many alternatives in this vital parameter.
  • Enhanced Stability and Biocompatibility
    The unique blend of DOTAP and Chol contributes to an enhanced stability and biocompatibility of these liposomes, making them a preferred choice for sensitive biomedical applications.
  • Optimal Size for Cellular Uptake
    With a carefully controlled size range, these liposomes demonstrate optimal cellular uptake, crucial for effective drug and gene delivery applications.
  • Advanced Drug Loading Capability
    The incorporation of cholesterol in the liposomes not only stabilizes the bilayer but also facilitates the loading of hydrophilic drugs, offering an advanced platform for drug delivery systems.
  • Ideal for Gene Delivery
    Their cationic nature makes these liposomes particularly suitable for gene delivery, efficiently forming complexes with anionic nucleic acids and ensuring successful delivery into target cells.

Click the button below to contact us or submit your feedback about this product.

For Research Use Only. Not For Clinical Use

Online Inquiry