DOTAP is a positively charged molecule. The zeta potential of the liposomes depending on the molar percentage of matrix lipid (DOPC) and DOTAP in the liposome formulation.
Lipid Composition
DOTAP DOPC
Applications
Control liposomes, Membrane biology
Format
Liquid
Concentration
10mg/mL
Hydration Solution
PBS, pH 7.4
External Solution
PBS, pH 7.4
Storage
2°C - 8°C (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Dosage
Download
DataSheet
MSDS
FAQsPublished Data Customer ReviewsRelated Sections
What are DOTAP:DOPC liposomes?
DOTAP:DOPC liposomes are cationic liposomes made from a mixture of DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) and DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine). This composition imparts a positive charge to the liposomes, making them suitable for binding and delivering negatively charged molecules like nucleic acids.
What advantages do DOTAP:DOPC liposomes offer for drug delivery?
The cationic nature of these liposomes enhances their interaction with negatively charged cellular membranes, facilitating more efficient cellular uptake. This property is particularly beneficial for the delivery of genetic materials such as DNA and RNA into cells.
How are DOTAP:DOPC liposomes typically used in research?
These liposomes are commonly used in gene therapy research for the delivery of DNA, RNA, and siRNA to target cells. Their ability to form stable complexes with nucleic acids enhances the efficiency of gene transfer.
Can DOTAP:DOPC liposomes encapsulate other types of molecules?
Yes, apart from nucleic acids, these liposomes can also encapsulate proteins, peptides, and small molecule drugs. Their versatile nature makes them suitable for various therapeutic applications.
What are the typical physical properties of DOTAP:DOPC liposomes?
These liposomes generally have a mean particle size 100 nm. This size is ideal for ensuring good stability and optimal interaction with target cells.
Fluorescence confocal images of representative GUVs composed of DOPC incubation with SUVs of (A) DOTAP:DOPC (0% DOTAP), (B) DOTAP:DOPC (15% DOTAP), (C) DOTAP:DOPC (30% DOTAP).
This study aims to develop high-fusogenic liposomes with minimal toxicity. Researchers co-incubated DOTAP:DOPC SUVs (labeled with red fluorescence) with DOPC GUVs (labeled with green fluorescence) to assess the SUVs' ability to fuse with different membranes. According to fluorescence confocal images, SUVs lacking DOTAP showed no fusion with GUVs. However, increasing the DOTAP concentration to 15% or 30% resulted in the observation of mixed red and green fluorescence on GUVs. These findings suggest that adding DOTAP to SUVs increases their fusogenicity, with fusogenicity levels increasing as the proportion of DOTAP in the SUV composition increases. The results of this work may be used to develop cationic liposomes that are extremely fusogenic while yet being somewhat safe.
Wang, Yifei, et al. "On fusogenicity of positively charged phased-separated lipid vesicles: experiments and computational simulations." Biomolecules 13.10 (2023): 1473. Under Open Access license CC BY 4.0, the image was edited from figure 4.
Superior Nucleic Acid Delivery
These liposomes provide superior nucleic acid delivery, greatly improving our RNA-based therapeutic studies.
Creative Biolabs' Robust Formulations
Creative Biolabs' precise formulation of DOTAP:DOPC liposomes ensures high stability and functionality in our drug delivery research.
Optimal for Membrane Fusion Studies
The composition of these liposomes makes them optimal for studying membrane fusion, essential in our cell biology research.
Effective in Immunotherapy Applications
The DOTAP:DOPC liposomes are highly effective in delivering immunotherapeutic agents, showing promising results in our clinical trials.
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For Research Use Only. Not For Clinical Use 
