DOTAP:DOPE liposomes are primarily used for delivering genetic materials like DNA, RNA, siRNA, and miRNA. They're effective in gene therapy applications due to their ability to efficiently encapsulate nucleic acids and facilitate cellular uptake.
The cationic nature of DOTAP allows these liposomes to interact electrostatically with the negatively charged nucleic acids. This interaction helps in effectively condensing and protecting the genetic material during delivery to cells.
The mean particle size of these liposomes is generally around 70-120 nm, making them suitable for various biomedical applications.
Yes, customization options are available. Researchers can request specific sizes, lipid compositions, or buffer conditions to suit their experimental needs. There are also services for encapsulating various types of molecules, such as proteins, peptides, vitamins, and minerals.
When handling these liposomes, it's important to maintain sterile conditions to avoid contamination. Also, avoid freezing the liposomes as it can affect their structural integrity. If aliquoting is necessary, it should be done under sterile conditions.
Interaction and fusion of liposomes, with and without DSPE-PEG, with clinically relevant bacteria.
This study explores the delivery of oligonucleotides (ONs) to gram-positive and gram-negative Bacteria using DOTAP:DOPE liposomes. The interaction and fusion of liposomes and bacterial envelopes form the foundation for delivering ONs into bacteria. The interaction and fusion of DOTAP:DOPE liposomes (PEGylated and non-PEGylated) with Escherichia coli K12, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus Mu50, and Enterococcus faecium were studied using epifluorescence microscopy and lipid-mixing assays. The researchers observed that non-PEGylated liposomes showed homogenous cell staining, but PEGylated liposomes showed a halo around the cells. Although PEGylation can improve liposome stability, it also limits the electrostatic and fusion interaction between cationic liposomes and negatively charged bacterial membranes. This study shows that non-PEGylated DOTAP:DOPE liposomes can serve as an effective platform for delivering ONs into bacteria to overcome antibiotic resistance.
Pereira, Sara, et al. "Lipoplexes to deliver oligonucleotides in gram-positive and gram-negative bacteria: Towards treatment of blood infections." Pharmaceutics 13.7 (2021): 989. Under Open Access license CC BY 4.0, without modification.
Click the button below to contact us or submit your feedback about this product.
Online Inquiry