DOTAP:DOPE Liposomes (CAT#: LDLY-0123-LY170)

Description
Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DOTAP
DOPE
Applications
Cancer research; Target delivery
Format
Liquid
Concentration
5 mM
Hydration Solution
UltraPure Distilled Water
External Solution
UltraPure Distilled Water
Storage
2°C - 8°C (do not freeze)
Size
70-120 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Download
DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. What are the main applications of DOTAP:DOPE liposomes?

    DOTAP:DOPE liposomes are primarily used for delivering genetic materials like DNA, RNA, siRNA, and miRNA. They're effective in gene therapy applications due to their ability to efficiently encapsulate nucleic acids and facilitate cellular uptake.

  2. How do DOTAP:DOPE liposomes interact with genetic materials?

    The cationic nature of DOTAP allows these liposomes to interact electrostatically with the negatively charged nucleic acids. This interaction helps in effectively condensing and protecting the genetic material during delivery to cells.

  3. What is the article size of these liposomes?

    The mean particle size of these liposomes is generally around 70-120 nm, making them suitable for various biomedical applications.

  4. Can DOTAP:DOPE liposomes be customized for specific research needs?

    Yes, customization options are available. Researchers can request specific sizes, lipid compositions, or buffer conditions to suit their experimental needs. There are also services for encapsulating various types of molecules, such as proteins, peptides, vitamins, and minerals.

  5. Are there any precautions or special handling requirements?

    When handling these liposomes, it's important to maintain sterile conditions to avoid contamination. Also, avoid freezing the liposomes as it can affect their structural integrity. If aliquoting is necessary, it should be done under sterile conditions.

DOTAP:DOPE (50:50) Liposomes-fig1


Interaction and fusion of liposomes, with and without DSPE-PEG, with clinically relevant bacteria.

This study explores the delivery of oligonucleotides (ONs) to gram-positive and gram-negative Bacteria using DOTAP:DOPE liposomes. The interaction and fusion of liposomes and bacterial envelopes form the foundation for delivering ONs into bacteria. The interaction and fusion of DOTAP:DOPE liposomes (PEGylated and non-PEGylated) with Escherichia coli K12, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus Mu50, and Enterococcus faecium were studied using epifluorescence microscopy and lipid-mixing assays. The researchers observed that non-PEGylated liposomes showed homogenous cell staining, but PEGylated liposomes showed a halo around the cells. Although PEGylation can improve liposome stability, it also limits the electrostatic and fusion interaction between cationic liposomes and negatively charged bacterial membranes. This study shows that non-PEGylated DOTAP:DOPE liposomes can serve as an effective platform for delivering ONs into bacteria to overcome antibiotic resistance.

Pereira, Sara, et al. "Lipoplexes to deliver oligonucleotides in gram-positive and gram-negative bacteria: Towards treatment of blood infections." Pharmaceutics 13.7 (2021): 989. Under Open Access license CC BY 4.0, without modification.

  • Innovative Delivery for Genetic Material
    The DOTAP:DOPE liposomes from Creative Biolabs proved to be remarkably efficient in gene delivery. The ease of complexing with siRNA and the impressive hydrodynamic diameter measurements show their superiority in nucleic acid delivery applications.
  • A Game-Changer in Cancer Research
    Utilizing these liposomes in my ovarian cancer cell line research, I noticed a substantial improvement in transfection efficiency. The unique lipid composition significantly enhanced cellular uptake of therapeutic agents.
  • Exceptional Stability and Efficiency
    Creative Biolabs' DOTAP:DOPE liposomes show unparalleled stability in protein corona composition regardless of centrifugation factors. This stability is a game changer in drug delivery research, setting it apart from other products.
  • Versatile Application Range
    Whether for low or high protein concentration studies, Creative Biolabs' liposomes maintain consistent efficiency. This versatility is essential for diverse research applications, providing an edge over competitors.
  • Superb for Hepatoma Cell Studies
    Working with hepatoma cell lines, the DOTAP:DOPE liposomes from Creative Biolabs provided a noticeable boost in gene transfection efficiency. Their consistent performance makes them invaluable for my ongoing research.

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For Research Use Only. Not For Clinical Use

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