DOTAP Liposomes (CAT#: LDLY-0123-LY171)

Description
Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DOTAP
Applications
Cancer research; Target delivery
Format
Liquid
Hydration Solution
Deionized RNAse-free Water
External Solution
Deionized RNAse-free Water
Storage
2°C - 8°C (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Download
DataSheet MSDS
FAQs Customer Reviews Related Sections
  1. What are DOTAP liposomes and their primary uses?

    DOTAP liposomes are cationic liposomes, mainly utilized in transfection applications for delivering genetic materials like DNA and RNA into cells. They are particularly effective in transfecting various cell types, including primary and suspension cells, and have applications in both in vitro and in vivo settings.

  2. What are the key considerations in preparing DOTAP liposome formulations for use in experiments?

    When preparing DOTAP liposome formulations, it's important to consider the lipid-to-DNA ratio, as this can significantly affect transfection efficiency. The pH and ionic strength of the buffer used can also influence the complex formation and stability.

  3. Can DOTAP liposomes be used in conjunction with other compounds or agents?

    Yes, DOTAP liposomes can be used with other compounds. However, interactions between the liposomes and these agents must be evaluated to ensure they do not negatively affect the liposomes' integrity or the overall efficacy of the delivery system.

  4. Are there specific cell types or conditions where DOTAP liposomes demonstrate optimal efficiency?

    DOTAP liposomes are generally versatile but may exhibit varying efficiencies depending on the cell type. They are particularly effective in transfecting hard-to-transfect cells. The specific conditions, such as cell density and health, can also impact the transfection efficiency.

  5. How can one assess the success of transfection when using DOTAP liposomes?

    The success of transfection can be assessed by analyzing the expression of the delivered genetic material, using methods like quantitative PCR, Western blotting, or fluorescence microscopy, depending on the nature of the transfected material.

  • Exceptional Delivery System for Gene Therapy
    The DOTAP liposomes from Creative Biolabs have proven invaluable in our lab's gene therapy research. Their superior delivery efficiency greatly enhances gene transfection, a critical step in advancing our therapeutic strategies.
  • Impressive Performance in Immunization Studies
    In our immunization experiments, Creative Biolabs' DOTAP liposomes demonstrated a remarkable capacity to boost antigen-specific immune responses. Their effectiveness in mucosal adjuvant activity was particularly noteworthy, enabling significant advances in our vaccine development projects.
  • Robust and Reliable for Biomedical Research
    For anyone engaged in biomedical research, Creative Biolabs' DOTAP liposomes are a must-have. Their reliability and high performance in various applications, from drug delivery to gene therapy, have been consistently impressive in our lab's rigorous testing.
  • Advanced Tool for Drug Delivery Systems
    In the area of targeted drug delivery, Creative Biolabs' DOTAP liposomes are able to effectively encapsulate and release therapeutic agents due to their unique composition, significantly enhancing the efficacy of our drug delivery systems.
  • Optimal for Nucleic Acid Delivery
    We've found the DOTAP liposomes from Creative Biolabs to be exceptionally effective for nucleic acid delivery. Their ability to facilitate the transfer of DNA and RNA into target cells has been instrumental in our genetic research, outperforming other products on the market.

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For Research Use Only. Not For Clinical Use

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