GL67:DOPE Liposomes (CAT#: LDLY-0123-LY206)

GL67:DOPE liposomes are formulated with GL67, a cationic lipid, and DOPE (Dioleoylphosphatidylethanolamine). This combination creates liposomes with a positive charge, optimizing them for efficient delivery of nucleic acids by forming stable complexes through electrostatic interaction.

For optimal stability, GL67:DOPE liposomes should be stored in the dark at 4°C. They are prepared under sterile conditions, and care should be taken to avoid contamination when accessing them for multiple uses​.

GL67, known as N^4-cholesteryl-spermine HCl salt, is a cholesterol derivative combined with spermine to form a cationic lipid. It has shown efficacy in mediating gene transfer into mammalian cells, highlighted by its key role in clinical studies for delivering plasmid DNA (pDNA) via inhalation, demonstrating its potential for gene therapy applications.

GL67:DOPE liposomes have been utilized in various gene therapy research, including delivering therapeutic genes to treat diseases such as cystic fibrosis and muscular dystrophy. Their ability to efficiently deliver DNA and RNA molecules to a range of cell types, including cancer and immune cells, underlines their versatility in drug development and research applications​.

Optimizing nucleic acid loading into GL67:DOPE liposomes involves adjusting the ratio of liposome to nucleic acid to ensure maximum complexation without compromising the stability of the liposome structure. It's essential to start with a low ratio and incrementally adjust while monitoring the efficiency of gene delivery and cytotoxicity in vitro. Preliminary experiments to determine the optimal conditions, including incubation time and temperature, can significantly enhance transfection efficiency.