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Magic™ Antibody Discovery - Human CD3 epsilon&CD3 gamma Heterodimer (23-126(CD3E)&23-116(CD3G)) Membrane Protein, Partial, -hIgG1 Fc -His tag & -hIgG1 Fc -Flag tag (CAT#: MP1283F)

Datasheet
This membrane protein is Human CD3 epsilon&CD3 gamma Heterodimer (23-126(CD3E)&23-116(CD3G)). It has been tested in SDS-PAGE, ELISA, SEC-SEC-MALS, SPR. We provide this protein to facilitate your membrane protein antibody discovery and development.

Product Specifications

  • Host Species
  • Human
  • Target Protein
  • CD3 epsilon&CD3 gamma Heterodimer
  • Protein Length
  • ECD
  • Molecular Weight
  • Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.
  • Sequence
  • AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1(CD3E) & P09693-1(CD3G)).

Product Description

  • Activity
  • Yes
  • Application
  • SDS-PAGE, ELISA, SEC-SEC-MALS, SPR
  • Expression Systems
  • HEK293
  • Tag
  • CD3E is fused with a Human IgG1 Fc tag at the C-terminus, followed by His tag, and subunit CD3G is fused with a Human IgG1 Fc tag and Flag tag at the C-terminus.
  • Protein Format
  • Soluble
  • Reconstitution
  • Please see Certificate of Analysis for specific instructions.
  • Endotoxin
  • <1.0 EU/μg by the LAL method
  • Purity
  • >95% as determined by SDS-PAGE.
    >90% as determined by SEC-MALS.
  • Buffer
  • Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Target

  • Target Protein
  • CD3 epsilon&CD3 gamma Heterodimer
  • Full Name
  • CD3e molecule&CD3g molecule
  • Introduction
  • The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
  • Alternative Names
  • CD3-epsilon
    CD3e antigen, epsilon polypeptide (TiT3 complex)
    CD3e molecule, epsilon (CD3-TCR complex)
    T-cell antigen receptor complex, epsilon subunit of T3
    T-cell surface antigen T3/Leu-4 epsilon chain, CD3g antigen, gamma polypeptide (TiT3 complex)
    CD3g molecule, epsilon (CD3-TCR complex)
    CD3g molecule, gamma (CD3-TCR complex)
    T-cell antigen receptor complex, gamma subunit of T3
    T-cell receptor T3 gamma chain

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