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Magic™ Antibody Discovery - Human IL-12B&IL-12A Heterodimer (23-328(IL-12B)&23-219(IL-12A)) Membrane Protein, Partial, -His tag & -Flag tag (CAT#: MP0237F)

Datasheet
This membrane protein is Human IL-12B&IL-12A Heterodimer (23-328(IL-12B)&23-219(IL-12A)). It has been tested in SDS-PAGE, ELISA, BLI, SPR. We provide this protein to facilitate your membrane protein antibody discovery and development.

Product Specifications

  • Host Species
  • Human
  • Target Protein
  • IL-12B&IL-12A Heterodimer
  • Protein Length
  • ECD
  • Molecular Weight
  • Calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). The reducing (R) heterodimer protein migrates as 43 kDa and 45 kDa (IL12B) and 35-40 kDa (IL12A) respectively due to glyosylation.
  • Sequence
  • AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1(IL12B) & P29459-1(IL12A)).

Product Description

  • Activity
  • Yes
  • Application
  • SDS-PAGE, ELISA, BLI, SPR
  • Expression Systems
  • HEK293
  • Tag
  • IL-12B is fused with His tag at the C-terminus. and subunit IL-12A is fused with Flag tag at the C-terminus.
  • Protein Format
  • Soluble
  • Reconstitution
  • Please see Certificate of Analysis for specific instructions.
  • Endotoxin
  • <1.0 EU/μg by the LAL method
  • Purity
  • >90% as determined by SDS-PAGE.
  • Buffer
  • Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Target

  • Target Protein
  • IL-12B&IL-12A Heterodimer
  • Full Name
  • interleukin 12B&interleukin 12A
  • Introduction
  • This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.&This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
  • Alternative Names
  • CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B; interleukin-12 subunit beta; CLMF p40; IL-12 subunit p40; IL12, subunit p40; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; natural killer cell stimulatory factor, 40 kD subunit&P35; CLMF; NFSK; NKSF1; IL-12A; interleukin-12 subunit alpha; CLMF p35; IL-12, subunit p35; IL35 subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain

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