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This membrane protein is Mouse EGF (977-1029). It has been tested in SDS-PAGE, ELISA, SPR. We provide this protein to facilitate your membrane protein antibody discovery and development.
Product Specifications
Host Species
Mouse
Target Protein
EGF
Protein Length
ECD
Molecular Weight
The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Sequence
AA Asn 977 - Arg 1029 (Accession # P01132-1).
Product Description
Activity
Yes
Application
SDS-PAGE, ELISA, SPR
Expression Systems
HEK293
Tag
Human IgG1 Fc tag at the N-terminus
Protein Format
Soluble
Reconstitution
Please see Certificate of Analysis for specific instructions.
Endotoxin
<1.0 EU/μg by the LAL method
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Target
Target Protein
EGF
Full Name
epidermal growth factor
Introduction
This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis.