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Magic™ BCR Repertoire Profiling for SHM Patterns Analysis

Creative Biolabs offers the unparalleled mass sequencing service to analyze the B cell receptors (BCR) repertoires. Our scientists have employed a novel strategy to develop next-generation targeting models for SHM patterns analysis. Our models will provide insights into the SHM process and support analysis of mutation patterns. Creative Biolabs is proud to introduce this high-quality service to our global clients.

The Background of SHM Patterns

From the preimmune repertoire, BCR molecules usually bind antigens with only modest affinity and specificity. Fine tuning of the antibody response is driven by another lymphocyte-specific process known as SHM. SHM is a cellular mechanism by which the immune system confronts a new foreign antigen. In the SHM process, BCR genes are diversified by introducing point mutations into immunoglobulin (Ig) genes at a high rate. Although stochastic, SHM is biased by the local DNA sequence context and preferentially introduces mutations at specific DNA motifs (hot spots) while avoiding others (cold spots). SHM is critical for the generation of high-affinity antibodies and effective immune responses. Statistical analysis of SHM patterns often requires background models of SHM. The SHM process can be quantitatively characterized by a targeting mode. It can improve the ability to detect deviations in SHM pathways related to diseases and identify selected mutations that drive Ag specificity and affinity maturation. Analyses of somatic hypermutation (SHM) patterns have important basic science and clinical applications. Previous work has focused on studying mutations in Ig sequences. However, the small sequence database and short motif comparisons limited the resolution of this analysis. Modeling these intrinsic biases has been limited by the lack of large sets of Ig sequences, driving the need for accurate inherent SHM properties in the absence of Ag-driven selection.

SHM as a mechanism of B cell epitope spreading Fig.1 SHM as a mechanism of B cell epitope spreading (Cornaby et al. 2015).

SHM Patterns Analysis in Creative Biolabs

Combined with our Magic™ next-generation sequencing platform, Creative Biolabs has employed a novel strategy to span the full V gene to develop next-generation targeting model for SHM patterns analysis. These models can accurately characterize the inherent SHM properties in the absence of Ag-driven selection. The process consists of (1) cell sorting, (2) sequencing, (3) quality control, and (4) construction of a sequencing-error model and a quantitative SHM targeting model. In the targeting model, targeting biases are consistent with classic hot and cold spots, and moreover, it can reveal additional highly mutable motifs.

Key Advantages of SHM Patterns Analysis Service


Equipped with world-leading technology platforms and professional scientific staff in the field of immunology, scientists of Creative Biolabs are proficient in developing next-generation targeting models for SHM patterns analysis. These models can accurately characterize the inherent SHM properties in the absence of Ag-driven selection. We are pleased to offer the best service with the most accurate results for our global customers.

Please contact us for more information and a detailed quote.

Reference

  1. Cornaby, C.; et al. B cell epitope spreading: mechanisms and contribution to autoimmune diseases. Immunol Lett. 2015, 163(1):56-68.

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