As a pioneer and undisputed global leader in immune repertoire analysis, Creative Biolabs has developed a Magic™ platform for large-scale sequencing service. Our scientists have employed a powerful strategy to identify the vast majority of BCR V(D)JC sequences of B-cell receptor (BCR) repertoire in canine. We are more than happy to share our experience in canine BCR repertoire analysis to facilitate their meaningful immune repertoire projects.
Canine BCR Repertoire Properties
Complementarity determining regions (CDRs) are responsible for binding antigen and provide substantial diversity to the BCR repertoire. VH CDR3 of the immunoglobulin variable heavy (VH) domain plays a dominant role in immune response. The canine repertoire is heavily dominated by IGHV1 subgroup, which is evolutionarily close to the human IGHV3 subgroup. The productive canine repertoire shows many similarities to both human and mouse. However, there are distinct differences in terms of VH CDR3 length and amino acid paratope composition. Compared to human and murine antibody repertoire, the length of canine VH CDR3 regions is longer than the murine VH CDR3 but shorter than the human counterparts. The amino acids at the base of the VH CDR3 loop are strictly conserved, which is similar to corresponding human and mouse VH CDR3. Moreover, there are significant changes in chemical paratope composition in CDR positions. Furthermore, canine VH CDR3 displays an overrepresentation of the neutral amino acid threonine and the negatively charged aspartic acid while proline content is similar to that in the human repertoire. Overall the canine repertoire shows a bias towards small, negatively charged amino acids.
Fig.1 VH CDR3 length distribution of canine breeds and comparison with mouse/human repertoires (Steiniger et al. 2014).
Canine BCR Repertoire Profiling in Creative Biolabs
Through our powerful Magic™ platform, we can provide one-stop service including high-throughput sequencing and data analysis. PBMC, bone marrow or spleen, or RNA from them can be good starting materials as the template for the amplification of CDR. Both the heavy chain and the light chain CDR 3 regions of canine can be 100% covered and accurately sequenced. We can offer our customers comprehensive BCR repertoire analysis, including hypermutation and isotype data.
Key Advantages of Canine BCR Repertoire Analysis Service
Creative Biolabs is one of the well-recognized experts and we are professional in BCR repertoire analysis in canine. With years of experience, our scientists can offer high-quality service of canine BCR repertoire analysis. We are pleased to use our extensive experience and advanced platform to offer the best service of BCR repertoire profiling in canine to satisfy each demand from our customers.
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