Close
Close

Magic™ Cell-free Membrane Protein Expression in Human Hela Cell Lysate (CAT#: MPCFK-2-2)

Datasheet
Magic™ Cell-free Protein Expression in Human Hela Cell Lysate Kit is a mammalian in vitro expression system based on HeLa cell lysates. The kit contains all factors required for protein synthesis including ribosomes, initiation factors, elongation factors and tRNA. Reactions are started by mixing plasmid DNA with the reaction mix. This system can synthesize protein for up to 6 hours.
Magic™ Cell-free Protein Expression in Human Hela Cell Lysate Kit is an optimized kit contains a T7 promoter and an EMCV internal ribosome entry site (IRES) to facilitate high levels of in vitro protein expression. Using a vector containing the EMCV IRES element is critical for obtaining high expression levels in this human in vitro protein expression system.

Product Specifications

  • Lysate Type
  • Human Hela Cell Lysate
  • Size
  • 8 reactions; 40 reactions
  • Reaction Volume
  • 25 µL per reaction
  • Expected Yield of Positive Control
  • 0.1 mg/ml
  • Components
  • HeLa Cell Extract
    Accessory Proteins
    Reaction Mix
    Nuclease-free Water
    Positive Control DNA (0.5µg/µL)
    Expression Vector (0.5µg/µL)
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

High protein yield

The long protein synthesis time ensures an abundant protein yield.
2023.1.06

Q&As

What are the important steps for successful protein expression and purification using a two-step in vitro human cell-free expression system?

1) successful amplification and cloning of genes into pT7CFE-CHis plasmid vector; 2) transcribing RNA at 30oC; 3) translation of protein at 30 °C in the presence of RNAse inhibitor; 4) developing the affinity based purification protocol using resin beads coated with Ni and 5) analyzing results on western blot using polyclonal and monoclonal antibodies.
2023.01.10

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Related Products
Online Inquiry
CONTACT US
USA:
Europe:
Germany:
Call us at:
USA:
UK:
Germany:
Fax:
Email:
Our customer service representatives are available 24 hours a day, 7 days a week. Contact Us
© 2024 Creative Biolabs. | Contact Us