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Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate 15N His-tagged Protein Labeling for NMR (CAT#: MPCFK-3-18)

Magic™ Cell-free Protein Expression in Wheat Germ lysate 15N His-tagged Protein Labeling for NMR Kit is a cell-free protein synthesis system using wheat germ extracts for protein labeling. It contains 1 ml optimized and high-performance wheat germ extract for different reaction scales. The lysate removed wheat proteins that would otherwise bind to Ni resins during the purification step. The reaction buffer contains stable isotope 15N labeled amino acids.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • Different reaction scales
  • Reaction Volume
  • Different reaction scales
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • Wheat Germ Extracts
    40x 15N Reaction buffer component 1
    40x 15N Reaction buffer component 2
    40x 15N Reaction buffer component 3
    40x 15N Reaction buffer component 4
    Creatine Kinase
    5×Transcription Reagent
    NTPmix
    RNase Inhibitor
    SP6 RNA Polymerase
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

Performed well in 2D NMR experiments

Protein-ligand interactions can be accurately captured.
2023.1.25

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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