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Magic™ Cell-free Membrane Protein Expression in Wheat Germ lysate for Disulfide Bond Protien (CAT#: MPCFK-3-13)

Magic™ Cell-free Protein Expression in Wheat Germ lysate for Disulfide Bond Protien is suitable for production of proteins having disulfide bonds. By adding the human Protein disulfide isomerase and endoplasmic reticulum, oxidoreductin-1α, this kit allows to perform transcription and translation reactions to express proteins having disulfide bonds in the wheat germ cell-free expression system. Moreover, we have omitted DTT from reaction buffer to stabilize the disulfide bonds.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • 8 reactions
  • Reaction Volume
  • 226 µL per reaction
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • pEU-E01-MCS
    pEU-E01-His-tPA(297-562)(Positive Control)
    Wheat Germ Extracts
    DTT-free Reaction buffer
    SPU PCR sense primer
    SP6 PCR sense primer
    Transcription Reagent
    PDI & Ero1α mix
    Aluminum seal
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

The disulfide bonds in proteins are very stable

The expression of proteins containing disulfide bonds is very accurate and the results are stable.
2023.1.20

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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